Western Blot (WB)
(Figure 1 Western Blot Validation with SARS-CoV-2 Spike Recombinant Protein Loading: 50 ng per lane of SARS-CoV-2 Spike S1 recombinant protein. Antibodies: SARS-CoV-2 Spike 9091, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 μg/mL, Lane 2: 2 μg/mL)
Rabbit COVID 19 Spike (cleavage site) Coronavirus Polyclonal Antibody | anti-COVID-19 antibody
SARS-CoV-2 (COVID-19) Spike Antibody (cleavage site)
Gene Names
S; spike glycoprotein
Reactivity
Virus
Applications
ELISA, Immunofluorescence, Western Blot
Purity
Affinity chromatography purified via peptide column.
Synonyms
COVID 19 Spike (cleavage site) Coronavirus; Polyclonal Antibody; SARS-CoV-2 (COVID-19) Spike Antibody (cleavage site); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike (cleavage site); anti-COVID-19 antibody
Host
Rabbit
Reactivity
Virus
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid. Supplied in PBS containing 0.02% sodium azide.
Concentration
1mg/mL (varies by lot)
Sequence Length
1273
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes
WB: 0.5-4ug/mL
IF: 20ug/mL.
Antibody validated: Western Blot in human samples; Immunofluorescence in human samples. SARS-CoV-2 (COVID-19) Spike Antibody (cleavage site) can be used for the detection of SARS-CoV-2 (COVID-19) Spike protein in ELISA. It will detect 4ng of free peptide at 1ug/mL. All other applications and species not yet tested.
IF: 20ug/mL.
Antibody validated: Western Blot in human samples; Immunofluorescence in human samples. SARS-CoV-2 (COVID-19) Spike Antibody (cleavage site) can be used for the detection of SARS-CoV-2 (COVID-19) Spike protein in ELISA. It will detect 4ng of free peptide at 1ug/mL. All other applications and species not yet tested.
Immunogen
Anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Spike antibody was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 (COVID-19, 2019-nCoV) Spike glycoprotein.
The immunogen is located within 650-700 amino acids of SARS-CoV-2 (COVID-19, 2019-nCoV) Spike protein.
The immunogen is located within 650-700 amino acids of SARS-CoV-2 (COVID-19, 2019-nCoV) Spike protein.
Homology
Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (25%)
Isoforms
SARS-CoV-2 (COVID-19, 2019-nCoV) Spike has one isoform (1273aa).
Conjugation
Unconjugated
Preparation and Storage
Can be stored at 4 degree C for three months. Store at -20 degree C, stable for up to one year.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Figure 1 Western Blot Validation with SARS-CoV-2 Spike Recombinant Protein Loading: 50 ng per lane of SARS-CoV-2 Spike S1 recombinant protein. Antibodies: SARS-CoV-2 Spike 9091, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 μg/mL, Lane 2: 2 μg/mL)
Western Blot (WB)
(Figure 1 Western Blot Validation with SARS-CoV-2 Spike Recombinant Protein Loading: 50 ng per lane of SARS-CoV-2 Spike S1 recombinant protein. Antibodies: SARS-CoV-2 Spike 9091, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 μg/mL, Lane 2: 2 μg/mL)
ELISA (EIA)
(Figure 2 ELISA Validation with SARS-CoV-2 Spike Recombinant Protein Antibodies: SARS-CoV-2 (COVID-19) Spike antibody 9091. A direct ELISA was performed using Spike S1 recombinant protein as coating antigen and the anti-SARS-CoV-2 (COVID-19) Spike antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.)
ELISA (EIA)
(Figure 2 ELISA Validation with SARS-CoV-2 Spike Recombinant Protein Antibodies: SARS-CoV-2 (COVID-19) Spike antibody 9091. A direct ELISA was performed using Spike S1 recombinant protein as coating antigen and the anti-SARS-CoV-2 (COVID-19) Spike antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000ng/mL.)
Related Product Information for anti-COVID-19 antibody
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019-20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a Spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Coronavirus invades cells through Spike (S) glycoproteins, a class I fusion protein. It is the major viral surface protein that coronavirus uses to bind to the human cell surface receptor. It also mediates the fusion of host and viral cell membrane, allowing the virus to enter human cells and begin infection (3). The spike protein is the major target for neutralizing antibodies and vaccine development (4). The protein modeling suggests that there is strong interaction between Spike protein receptor-binding domain and its host receptor angiotensin-converting enzyme 2 (ACE2), which regulate both the cross-species and human-to-human transmissions of COVID-19 (5). The recent study has shown that the SARS-CoV-2 spike protein binds ACE2 with higher affinity than SARS-CoV spike protein (6).
Product Categories/Family for anti-COVID-19 antibody
References
1) Gorbalenya. bioRxiv: 2020.
2) Hui et al. Int J Infect Dis. 2020;91:264-266.
3) Belouzard et al. Viruses. 2012;4(6):1011-33.
4) Lee et al. J Virol. 2006;80(8):4079-87.
5) Wan et al. J Virol. 2020.
6) Wrapp et al. Science. 2020.
2) Hui et al. Int J Infect Dis. 2020;91:264-266.
3) Belouzard et al. Viruses. 2012;4(6):1011-33.
4) Lee et al. J Virol. 2006;80(8):4079-87.
5) Wan et al. J Virol. 2020.
6) Wrapp et al. Science. 2020.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
surface glycoprotein
NCBI Official Symbol
S
NCBI Official Synonym Symbols
spike glycoprotein
NCBI Protein Information
surface glycoprotein
Research Articles on COVID-19
Similar Products
Product Notes
The COVID-19 (Catalog #AAA154651) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 (COVID-19) Spike Antibody (cleavage site) reacts with Virus and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 Spike (cleavage site) Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). WB: 0.5-4ug/mL IF: 20ug/mL. Antibody validated: Western Blot in human samples; Immunofluorescence in human samples. SARS-CoV-2 (COVID-19) Spike Antibody (cleavage site) can be used for the detection of SARS-CoV-2 (COVID-19) Spike protein in ELISA. It will detect 4ng of free peptide at 1ug/mL. All other applications and species not yet tested. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Spike (cleavage site) Coronavirus, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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