Western Blot (WB)
(Staining (2ug/ml) of mouse brain extracts (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat DAPK2 Polyclonal Antibody | anti-DAPK2 antibody
Goat anti-DAPK2 Antibody
Immunohistochemistry: Immunohistochemistry: In paraffin embedded Human Breast shows staining of the luminar side of the cell membranes of epithelial cells in the ducts. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 40-45kDa bands seen in mouse brain extracts. Recommended for use at 1-3ug/ml.
Western Blot (WB)
(Staining (2ug/ml) of mouse brain extracts (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (2ug/ml) of mouse brain extracts (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq, Jul 2008]
Uniprot Description
DAPK2: an ubiquitously expressed protein kinase of the DAPK family. Activated by Ca(2+)/calmodulin. Contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain. Overexpression induces apoptosis. Expressed at high levels in heart, lung and skeletal muscle.
Protein type: Protein kinase, CAMK; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CAMK group; DAPK family
Chromosomal Location of Human Ortholog: 15q22.31
Cellular Component: cytoplasm; cytoplasmic vesicle
Molecular Function: ATP binding; calmodulin binding; identical protein binding; protein binding; protein serine/threonine kinase activity
Biological Process: anoikis; apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of autophagy
Research Articles on DAPK2
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Product Notes
The DAPK2 dapk2 (Catalog #AAA420208) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-DAPK2 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's DAPK2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: Immunohistochemistry: In paraffin embedded Human Breast shows staining of the luminar side of the cell membranes of epithelial cells in the ducts. Recommended concentration, 2-4ug/ml. Western Blot: Approx 40-45kDa bands seen in mouse brain extracts. Recommended for use at 1-3ug/ml. Researchers should empirically determine the suitability of the DAPK2 dapk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KALHPRRRSS TS. It is sometimes possible for the material contained within the vial of "DAPK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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