Rabbit COVID 19 NSP8 Coronavirus Polyclonal Antibody | anti-COVID-19 antibody

SARS-CoV-2 (COVID-19) NSP8 Antibody

Cat. Num. AAA154666

• Reactivity: Virus
• Applications: ELISA, Western Blot
• Purity: SARS-CoV-2 (COVID-19) NSP8 Antibody is affinity chromatography purified via peptide column
• Synonyms: COVID 19 NSP8 Coronavirus; Polyclonal Antibody; SARS-CoV-2 (COVID-19) NSP8 Antibody; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; NSP8; anti-COVID-19 antibody

• Host: Rabbit
• Reactivity: Virus
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: SARS-CoV-2 (COVID-19) NSP8 Antibody is affinity chromatography purified via peptide column
• Form/Format: Liquid; SARS-CoV-2 (COVID-19) NSP8 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for anti-COVID-19 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 0.5ug/mL; ; Antibody validated: SARS-CoV-2 (COVID-19) NSP8 antibody can detect 2 ng of free peptide at 1ug/mL in ELISA.
• Immunogen: Anti-SARS-CoV-2 (COVID-19) NSP8 antibody (9167) was raised against a peptide corresponding to 14 amino acids near the center of SARS-CoV-2 (COVID-19) NSP8 protein.; ; The immunogen is located within 40-90 amino acids of SARS-CoV-2 (COVID-19) NSP8.
• Homology: Predicted reactivity based on immunogen sequence: SARS-CoV NSP8 protein: (100%)
• Conjugation: Unconjugated
• Preparation and Storage: SARS-CoV-2 (COVID-19) NSP9 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ELISA (EIA)

(Figure 1 ELISA Validation with SARS-CoV-2 (COVID-19) NSP8 Protein Antibodies: SARS-CoV-2 (COVID-19) NSP8 Antibody, 9167. A direct ELISA was performed using SARS-CoV-2 NSP8 recombinant protein (10-415) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP8 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL)

Western Blot (WB)

(Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) NSP8 ProteinLoading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP8 protein (10-415).Antibodies: SARS-CoV-2 (COVID-19) NSP8, 9167, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL)

Related Product Information for anti-COVID-19 antibody

Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019-20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP8 plays a role in viral RNA synthesis (3,4,5). Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, it may synthesize substantially longer products than oligonucleotide primers (6).

Product Categories/Family for anti-COVID-19 antibody

Infectious Disease; COVID-19

References

Gorbalenya. bioRxiv: 2020.
Hui et al. Int J Infect Dis. 2020;91:264-266.
Gao et. al Science 2020;0:0-0
Yin et. al Science 2020;0:0-0

NCBI and Uniprot Product Information

• NCBI GI #: 1826688925
• NCBI GeneID: 43740578