ELISA (EIA)
(Figure 1 ELISA test of chicken anti- COVID-19 spike S2 protein. Antibodies: SARS-CoV-2 (COVID-19, 2019-nCoV) Spike (S2) antibody (at dilutions from 1ug/ml to 0.025ug/ml). A direct ELISA was performed with purified COVID-19 Spike protein (S2) as coating antigen and the affinity purified anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Spike (S2) antibody as the primary antibody. Secondary: Rabbit anti-chicken IgY Fc HRP conjugate at 65ng/ml. For specificity comparison, COVID-19 spike protein (S1) and human IgG were compared for binding by the same method.)
Chicken COVID 19 Spike S2 Coronavirus Polyclonal Antibody | anti-COVID-19 antibody
Affinity purified SARS-CoV-2 (COVID-19, 2019-nCoV) Spike S2 Domain Antibody
Reactivity
Virus
Applications
ELISA
Purity
>95%
Synonyms
COVID 19 Spike S2 Coronavirus; Polyclonal Antibody; Affinity purified SARS-CoV-2 (COVID-19; 2019-nCoV) Spike S2 Domain Antibody; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike S2; anti-COVID-19 antibody
Host
Chicken
Reactivity
Virus
Clonality
Polyclonal
Isotype
IgY (IgG)
Purity/Purification
>95%
Form/Format
Liquid; PBS 0.01% Azide
Concentration
IgY derived from eggs at a concentration of 1 mg/ml in PBS with 0.01% azide. (varies by lot)
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA)
Application Notes
SARS-CoV-2 (COVID-19, 2019-nCoV) Spike antibody can be used for detection of full length SARS-CoV-2 (COVID-19, 2019-nCoV) Spike protein (S1+S2) or truncated SARS-CoV-2 (COVID-19, 2019-nCoV) spike protein S2 domain by ELISA.
Homology
Predicted reactivity based on immunogen sequence: SARSCoV Spike protein (S2):(100%)
Immunogen
Anti-SARS-CoV-2 (COVID-19, 2019-nCov) Spike antibody was raised against recombinant SARS-CoV-2 (2019-nCoV) spike protein (S2) domain.
The immunogen is the spike potein S2 domain produced using HEK293 cells.
The immunogen is the spike potein S2 domain produced using HEK293 cells.
Immunogen Note
Immunogen has been tested for activity and can bind with human ACE2 in functional ELISA assay.
Conjugate
Unconjugated
Sterile
0.2um filtration
Total Protein
100ug
Preparation and Storage
SARS-Cov-2 (COVID-19, 2019-nCoV) spike antibody can be stored at 4°C for one month. Aliquot and store at -20°C for long term (stable for up to one year). Avoid repeated freeze and thaw cycles.
ELISA (EIA)
(Figure 1 ELISA test of chicken anti- COVID-19 spike S2 protein. Antibodies: SARS-CoV-2 (COVID-19, 2019-nCoV) Spike (S2) antibody (at dilutions from 1ug/ml to 0.025ug/ml). A direct ELISA was performed with purified COVID-19 Spike protein (S2) as coating antigen and the affinity purified anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Spike (S2) antibody as the primary antibody. Secondary: Rabbit anti-chicken IgY Fc HRP conjugate at 65ng/ml. For specificity comparison, COVID-19 spike protein (S1) and human IgG were compared for binding by the same method.)
ELISA (EIA)
(Figure 1 ELISA test of chicken anti- COVID-19 spike S2 protein. Antibodies: SARS-CoV-2 (COVID-19, 2019-nCoV) Spike (S2) antibody (at dilutions from 1ug/ml to 0.025ug/ml). A direct ELISA was performed with purified COVID-19 Spike protein (S2) as coating antigen and the affinity purified anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Spike (S2) antibody as the primary antibody. Secondary: Rabbit anti-chicken IgY Fc HRP conjugate at 65ng/ml. For specificity comparison, COVID-19 spike protein (S1) and human IgG were compared for binding by the same method.)
Related Product Information for anti-COVID-19 antibody
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus, originated from the Wuhan region of Hubei province in China late in 2019 (1). Coronavirus invades cells through spike protein mediated binding human airway epithelial cell surface receptor Angiotensin converting enzyme 2 (ACE2). Spike protein also mediates fusion of host and viral cell membrane to begin infection (2). Spike protein is the major target for neutralizing antibodies and vaccine development (3).
The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain, which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.
Protein modeling suggests that there is a strong interaction between Spike protein receptor-binding domain and its host receptor angiotensin-converting enzyme 2 (ACE2), which regulate both the cross-species and human-to-human transmissions of COVID-19 (4). A recent study has shown that the SARS-CoV-2 spike protein binds ACE2 with higher affinity than SARS-CoV spike protein (5).
The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain, which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.
Protein modeling suggests that there is a strong interaction between Spike protein receptor-binding domain and its host receptor angiotensin-converting enzyme 2 (ACE2), which regulate both the cross-species and human-to-human transmissions of COVID-19 (4). A recent study has shown that the SARS-CoV-2 spike protein binds ACE2 with higher affinity than SARS-CoV spike protein (5).
References
1) Gorbalenya. bioRxiv: 2020.
2) Belouzard et al. Viruses. 2012;4(6):1011-33.
3) Lee et al. J Virol. 2006;80(8):4079-87.
4) Wan et al. J Virol. 2020.
5) Wrapp et al. Science. 2020.
2) Belouzard et al. Viruses. 2012;4(6):1011-33.
3) Lee et al. J Virol. 2006;80(8):4079-87.
4) Wan et al. J Virol. 2020.
5) Wrapp et al. Science. 2020.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The COVID-19 (Catalog #AAA378288) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Affinity purified SARS-CoV-2 (COVID-19, 2019-nCoV) Spike S2 Domain Antibody reacts with Virus and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 Spike S2 Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). SARS-CoV-2 (COVID-19, 2019-nCoV) Spike antibody can be used for detection of full length SARS-CoV-2 (COVID-19, 2019-nCoV) Spike protein (S1+S2) or truncated SARS-CoV-2 (COVID-19, 2019-nCoV) spike protein S2 domain by ELISA. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Spike S2 Coronavirus, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
