Immunohistochemistry (IHC)
(Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF3b in COVID-19 Patient LungImmunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) ORF3b antibody (9279, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF3b protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.)
Rabbit COVID 19 ORF3b Coronavirus Polyclonal Antibody | anti-COVID-19 antibody
SARS-CoV-2 (COVID-19) ORF3b Antibody
Reactivity
Virus
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
SARS-CoV-2 (COVID-19) ORF3b Antibody is affinity chromatography purified via peptide column.
Synonyms
COVID 19 ORF3b Coronavirus; Polyclonal Antibody; SARS-CoV-2 (COVID-19) ORF3b Antibody; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; ORF3b; ORF3b protein; Acessary protein 3b; ns3b; Non-structural protein 3b; anti-COVID-19 antibody
Host
Rabbit
Reactivity
Virus
Clonality
Polyclonal
Isotype
IgG
Specificity
ORF3b Antibody is predicted to not cross-react with other coronavirus family members.
Purity/Purification
SARS-CoV-2 (COVID-19) ORF3b Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid. SARS-CoV-2 (COVID-19) ORF3b antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 0.25ug/mL
IHC: 0.5ug/mL
Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) ORF3b antibody can detect 2ng of free peptide at 1ug/mL in ELISA. It can detect SARS-CoV-2 ORF3b recombinant protein by ELISA and WB.
IHC: 0.5ug/mL
Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) ORF3b antibody can detect 2ng of free peptide at 1ug/mL in ELISA. It can detect SARS-CoV-2 ORF3b recombinant protein by ELISA and WB.
Organism
Severe acute respiratory syndrome coronavirus 2
Immunogen
Anti-SARS-CoV-2 (COVID-19) ORF3b antibody was raised against a peptide corresponding to 16 amino acids near the central domain of SARS-CoV-2 (COVID-19) ORF3b protein.
The immunogen is located within 30-80 amino acids of the SARS-CoV-2 (COVID-19) ORF3b protein.
The immunogen is located within 30-80 amino acids of the SARS-CoV-2 (COVID-19) ORF3b protein.
Conjugation
Unconjugated
Protein GI#
1835921851
Preparation and Storage
SARS-CoV-2 (COVID-19) ORF3b antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Immunohistochemistry (IHC)
(Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF3b in COVID-19 Patient LungImmunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) ORF3b antibody (9279, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF3b protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.)
Immunohistochemistry (IHC)
(Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF3b in COVID-19 Patient LungImmunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) ORF3b antibody (9279, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF3b protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.)
ELISA (EIA)
(Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) ORF3b Protein Antibodies: SARS-CoV-2 (COVID-19) ORF3b Antibody, 9279. A direct ELISA was performed using SARS-CoV-2 ORF3b recombinant protein (10-005) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF3b antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL)
ELISA (EIA)
(Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) ORF3b Protein Antibodies: SARS-CoV-2 (COVID-19) ORF3b Antibody, 9279. A direct ELISA was performed using SARS-CoV-2 ORF3b recombinant protein (10-005) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF3b antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL)
Western Blot (WB)
(Figure 3 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF3b Protein</strong><br> Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) ORF3b recombinant protein. Antibodies: SARS-CoV-2 (COVID-19) ORF3b, 9279, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 μg/mL and Lane 2: 0.5 μg/mL)
Western Blot (WB)
(Figure 3 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF3b Protein</strong><br> Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) ORF3b recombinant protein. Antibodies: SARS-CoV-2 (COVID-19) ORF3b, 9279, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 μg/mL and Lane 2: 0.5 μg/mL)
Related Product Information for anti-COVID-19 antibody
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019-20 coronavirus outbreak (2). SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF3b, ORF9b, ORF9c and ORF10. ORF3b may play a role in interferon antiviral system evasion (3).
Product Categories/Family for anti-COVID-19 antibody
References
Gorbalenya. bioRxiv: 2020.
Hui et al. Int J Infect Dis. 2020;91:264-266.
Konno et al. Cell Rep. 2020; 32:108185.
Hui et al. Int J Infect Dis. 2020;91:264-266.
Konno et al. Cell Rep. 2020; 32:108185.
NCBI and Uniprot Product Information
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Product Notes
The COVID-19 (Catalog #AAA154763) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 (COVID-19) ORF3b Antibody reacts with Virus and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 ORF3b Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 0.25ug/mL IHC: 0.5ug/mL Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) ORF3b antibody can detect 2ng of free peptide at 1ug/mL in ELISA. It can detect SARS-CoV-2 ORF3b recombinant protein by ELISA and WB. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 ORF3b Coronavirus, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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