Immunohistochemistry (IHC)
(Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF7a in COVID-19 Patient LungImmunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) ORF7a antibody (1 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF7a protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.)
Rabbit COVID 19 ORF7a Coronavirus Polyclonal Antibody | anti-COVID-19 antibody
SARS-CoV-2 (COVID-19) ORF7a Antibody
Reactivity
VirusPredicted reactivity based on immunogen sequence: SARS-CoV ORF7a: (73%)
Applications
ELISA, Immunohistochemistry
Purity
SARS-CoV-2 (COVID-19) ORF7a Antibody is affinity chromatography purified via peptide column.
Synonyms
COVID 19 ORF7a Coronavirus; Polyclonal Antibody; SARS-CoV-2 (COVID-19) ORF7a Antibody; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; ORF7a; ORF7a protein; Accessory protein 7a; Protein U122; Protein X4; anti-COVID-19 antibody
Host
Rabbit
Reactivity
Virus
Predicted reactivity based on immunogen sequence: SARS-CoV ORF7a: (73%)
Predicted reactivity based on immunogen sequence: SARS-CoV ORF7a: (73%)
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
SARS-CoV-2 (COVID-19) ORF7a Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid. SARS-CoV-2 (COVID-19) ORF7a antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
IHC: 1ug/mL
Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) ORF7a antibody can detect 2ng of free peptide at 1ug/mL in ELISA.
Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) ORF7a antibody can detect 2ng of free peptide at 1ug/mL in ELISA.
Organism
Severe acute respiratory syndrome coronavirus 2
Immunogen
Anti-SARS-CoV-2 (COVID-19) ORF7a antibody was raised against a peptide corresponding to 15 amino acids near the central domain of SARS-CoV-2 (COVID-19) ORF7a protein.
The immunogen is located within 40-90 amino acids of the SARS-CoV-2 (COVID-19) ORF7a protein.
The immunogen is located within 40-90 amino acids of the SARS-CoV-2 (COVID-19) ORF7a protein.
Conjugation
Unconjugated
Protein GI#
1835921947
Preparation and Storage
SARS-CoV-2 (COVID-19) ORF7a antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Immunohistochemistry (IHC)
(Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF7a in COVID-19 Patient LungImmunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) ORF7a antibody (1 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF7a protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.)
Immunohistochemistry (IHC)
(Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF7a in COVID-19 Patient LungImmunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) ORF7a antibody (1 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF7a protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.)
ELISA (EIA)
(Figure 2 ELISA Validation Antibodies: SARS-CoV-2 (COVID-19) ORF7a Antibody, 9285. A direct ELISA was performed using SARS-CoV-2 ORF7a immunogen peptide (9285P)) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF7a antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL)
ELISA (EIA)
(Figure 2 ELISA Validation Antibodies: SARS-CoV-2 (COVID-19) ORF7a Antibody, 9285. A direct ELISA was performed using SARS-CoV-2 ORF7a immunogen peptide (9285P)) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF7a antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL)
Related Product Information for anti-COVID-19 antibody
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019-20 coronavirus outbreak (2). SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF8, ORF9b, ORF7a and ORF7a. ORF7a plays a role as antagonist of host tetherin (BST2), disrupting its antiviral effect. It acts by binding to BST2 thereby interfering with its glycosylation. It may suppress small interfering RNA (siRNA) and may bind to host ITGAL, thereby playing a role in attachment or modulation of leukocytes (3).
Product Categories/Family for anti-COVID-19 antibody
References
Gorbalenya. bioRxiv: 2020.
Hui et al. Int J Infect Dis. 2020;91:264-266.
Taylor et al. J. Virol. 2015; 89:11820-11833.
Hui et al. Int J Infect Dis. 2020;91:264-266.
Taylor et al. J. Virol. 2015; 89:11820-11833.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The COVID-19 (Catalog #AAA154766) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SARS-CoV-2 (COVID-19) ORF7a Antibody reacts with Virus Predicted reactivity based on immunogen sequence: SARS-CoV ORF7a: (73%) and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 ORF7a Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC). IHC: 1ug/mL Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) ORF7a antibody can detect 2ng of free peptide at 1ug/mL in ELISA. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 ORF7a Coronavirus, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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