Immunohistochemistry (IHC)
(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit anti-Human, Mouse PRPSAP1 Polyclonal Antibody | anti-PRPSAP1 antibody
Rabbit Polyclonal (IgG) to Human PRPSAP1
Gene Names
PRPSAP1; PAP39
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
PRPSAP1; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human PRPSAP1; Anti-PRPSAP1 Antibody (aa1-356) IHC-plus; PAP39; Human PRPSAP1; anti-PRPSAP1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human PRPSAP1
Purity/Purification
Immunoaffinity Purified
Form/Format
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Concentration
0.52 mg/ml (varies by lot)
Sequence Length
385
Applicable Applications for anti-PRPSAP1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes
IHC-P (5 ug/ml), WB (1:500 - 1:3000)
Usage: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Usage: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Target Species
Human
Immunogen Description
Recombinant fragment corresponding to a region within amino acids 1 and 356 of PRPSAP1 (SwissProt Q14558). Percent identity by BLAST analysis: Human (100%); Rat, Bovine (98%); Xenopus (86%).
Immunogen Type
Recombinant protein
Immunogen
PRPSAP1 antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 356 of PRPSAP1 (SwissProt Q14558). Percent identity by BLAST analysis: Human (100%); Rat, Bovine (98%); Xenopus (86%).
Antigen Modification
aa1-356
Preparation and Storage
Keep as concentrated solution. Aliquot and store at -20 degree C or below. Avoid multiple freeze-thaw cycles.
Immunohistochemistry (IHC)
(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
Western Blot (WB)
(Sample (30g whole cell lysate). A: HeLa S3, B: Hep G2. 10% SDS PAGE. PRPSAP1 antibody diluted at 1:1000)
Western Blot (WB)
(Sample (30g whole cell lysate). A: HeLa S3, B: Hep G2. 10% SDS PAGE. PRPSAP1 antibody diluted at 1:1000)
Western Blot (WB)
(Sample (50 ug of whole cell lysate). A: mouse liver. 10% SDS PAGE. PRPSAP1 antibody diluted at 1:1000.)
Western Blot (WB)
(Sample (50 ug of whole cell lysate). A: mouse liver. 10% SDS PAGE. PRPSAP1 antibody diluted at 1:1000.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,467 Da
NCBI Official Full Name
phosphoribosyl pyrophosphate synthase-associated protein 1
NCBI Official Synonym Full Names
phosphoribosyl pyrophosphate synthetase-associated protein 1
NCBI Official Symbol
PRPSAP1
NCBI Official Synonym Symbols
PAP39
NCBI Protein Information
phosphoribosyl pyrophosphate synthase-associated protein 1; PRPP synthase-associated protein 1; 39 kDa phosphoribosypyrophosphate synthase-associated protein
UniProt Protein Name
Phosphoribosyl pyrophosphate synthase-associated protein 1
Protein Family
UniProt Gene Name
PRPSAP1
UniProt Synonym Gene Names
PRPP synthase-associated protein 1; PAP39
UniProt Entry Name
KPRA_HUMAN
Uniprot Description
PRPSAP1: Seems to play a negative regulatory role in 5- phosphoribose 1-diphosphate synthesis. Belongs to the ribose-phosphate pyrophosphokinase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Inhibitor
Chromosomal Location of Human Ortholog: 17q24-q25
Molecular Function: ribose phosphate diphosphokinase activity; enzyme inhibitor activity; magnesium ion binding; kinase binding
Biological Process: negative regulation of kinase activity; nucleotide biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
Similar Products
Product Notes
The PRPSAP1 prpsap1 (Catalog #AAA248266) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal (IgG) to Human PRPSAP1 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRPSAP1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB). IHC-P (5 ug/ml), WB (1:500 - 1:3000) Usage: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0). Researchers should empirically determine the suitability of the PRPSAP1 prpsap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRPSAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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