Western Blot (WB)
(Staining (0.3ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat Coronin 1/TACO Polyclonal Antibody | anti-CORO1A antibody
Goat anti-Coronin 1/TACO Antibody
Western Blot: Approx 60kDa band observed in MOLT4 and Jurkat lysates (calculated MW of 51. 0 kDa according to NP_009005.1). In transfected HEK293 transiently expressing CORO1A band of approx.50kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.3-1ug/ml.
Western Blot (WB)
(Staining (0.3ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (0.3ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing CORO1A (RC210753) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing CORO1A (RC210753) and probed (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16. [provided by RefSeq, Sep 2010]
Uniprot Description
coronin 1A: May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria- infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. Belongs to the WD repeat coronin family.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: actin filament; axon; cortical actin cytoskeleton; cytoplasm; cytosol; early endosome; immunological synapse; intercellular junction; lamellipodium; membrane; nucleus; phagocytic cup; phagocytic vesicle; phagocytic vesicle membrane; plasma membrane; protein complex
Molecular Function: actin binding; actin filament binding; actin monomer binding; cytoskeletal protein binding; myosin heavy chain binding; phosphoinositide 3-kinase binding; protein binding; protein C-terminus binding; protein homodimerization activity
Biological Process: actin cytoskeleton organization and biogenesis; actin filament organization; calcium ion transport; cell motility; cell-substrate adhesion; formation of immunological synapse; homeostasis of number of cells within a tissue; innate immune response; leukocyte chemotaxis; natural killer cell degranulation; negative regulation of actin nucleation; negative regulation of neuron apoptosis; negative regulation of vesicle fusion; phagocytosis; phagolysosome formation; positive chemotaxis; positive regulation of cell migration; positive regulation of T cell proliferation; regulation of actin cytoskeleton organization and biogenesis; regulation of cell shape; regulation of release of sequestered calcium ion into cytosol; T cell homeostasis; uropod organization and biogenesis
Disease: Immunodeficiency 8
Research Articles on CORO1A
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Product Notes
The CORO1A coro1a (Catalog #AAA420501) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Coronin 1/TACO Antibody reacts with Tested: Humann Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Coronin 1/TACO can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 60kDa band observed in MOLT4 and Jurkat lysates (calculated MW of 51. 0 kDa according to NP_009005.1). In transfected HEK293 transiently expressing CORO1A band of approx.50kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.3-1ug/ml. Researchers should empirically determine the suitability of the CORO1A coro1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KRLDRLEETV QAK. It is sometimes possible for the material contained within the vial of "Coronin 1/TACO, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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