Rabbit CLTC Polyclonal Antibody | anti-CLTC antibody

Anti-CLTC Antibody, Rabbit Polyclonal

Cat. Num. AAA8103463

• Gene Names: Cltc; CHC; R74732; 3110065L21Rik
• Reactivity: Mouse, Human, Rat; Predicted to React with: Bovin (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: CLTC; Polyclonal Antibody; Anti-CLTC Antibody; Rabbit Polyclonal; CLTC Antibody; Rabbit PAb; Antigen Affinity Purified; anti-CLTC antibody

• Host: Rabbit
• Reactivity: Mouse, Human, Rat; Predicted to React with: Bovin (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse CLTC
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CLTC antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse CLTC
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse CLTC, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CLTC rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse brain tissue lysateLane B: HepG2 Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per laneSecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:180 kDaObserved band size:180 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining CLTC in mouse kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining CLTC in mouse adrenal gland with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining CLTC in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining CLTC in rat kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-CLTC antibody

The shared features of CLTC and other TFE3 fusion partners may shed light on the essential biology of TFE3 fusion proteins. CLTC, a scaffolding protein of clathrin-coated vesicles, in facilitating the fusion of autophagosomes with the CCV. During gene silencing of CLTC, CCVs are unable to fuse with each other, a phenotype also seen when silencing genes involved in macroautophagy/autophagy. Autophagosome fusion contributes to CCV size as cell starvation and subsequent autophagy induction leads to further CCV expansion. This is CLTC dependent, as the absence of CLTC renders autophagosomes no longer able to contribute to the expansion of the CCV.

NCBI and Uniprot Product Information

• NCBI GI #: 51491845
• NCBI GeneID: 67300
• NCBI Accession #: NP_001003908.1
• NCBI GenBank Nucleotide #: NM_001003908.1
• UniProt Accession #: Q68FD5
• Molecular Weight: 191,557 Da
• NCBI Official Full Name: clathrin heavy chain 1
• NCBI Official Synonym Full Names: clathrin, heavy polypeptide (Hc)
• NCBI Official Symbol: Cltc
• NCBI Official Synonym Symbols: CHC; R74732; 3110065L21Rik
• NCBI Protein Information: clathrin heavy chain 1
• UniProt Protein Name: Clathrin heavy chain 1
• Protein Family: Clathrin
• UniProt Gene Name: Cltc
• UniProt Entry Name: CLH1_MOUSE

Uniprot Description

CLTC: a major protein component of the cytoplasmic face of coated vesicles and coated pits. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.

Protein type: Motility/polarity/chemotaxis; Vesicle

Cellular Component: clathrin coat of trans-Golgi network vesicle; clathrin coat; focal adhesion; protein complex; mitochondrion; intracellular membrane-bound organelle; clathrin-coated vesicle; T-tubule; terminal button; coated pit; clathrin coat of coated pit; membrane coat; membrane; cytoplasm; spindle; cytoplasmic vesicle; sarcolemma; myelin sheath; vesicle

Molecular Function: protein C-terminus binding; protein binding; heat shock protein binding; double-stranded RNA binding; ankyrin binding; structural molecule activity; peptide binding; protein kinase binding; clathrin light chain binding

Biological Process: osteoblast differentiation; vesicle-mediated transport; mitosis; intracellular protein transport; receptor-mediated endocytosis; receptor internalization; transferrin transport; Golgi organization and biogenesis