Western Blot (WB)
(Anti-OTUB1 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per Lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:31 kDa Observed band size:34 kDa)
Rabbit OTUB1 Polyclonal Antibody | anti-OTUB1 antibody
Anti-OTUB1 Antibody, Rabbit Polyclonal
Gene Names
Otub1; AI850305
Reactivity
MousePredicted to React with: Human, Rat, Bovine (Species predicted to react based on 100% sequence homology)
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
OTUB1; Polyclonal Antibody; Anti-OTUB1 Antibody; Rabbit Polyclonal; OTUB1 Antibody; Rabbit PAb; Antigen Affinity Purified; OTU deubiquitinase; ubiquitin aldehyde binding 1; Anti-AI850305 Antibody; anti-OTUB1 antibody
Host
Rabbit
Reactivity
Mouse
Predicted to React with: Human, Rat, Bovine (Species predicted to react based on 100% sequence homology)
Predicted to React with: Human, Rat, Bovine (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse OTUB1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-OTUB1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthetic peptide corresponding to the center region of the Mouse OTUB1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse OTUB1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-OTUB1 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per Lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:31 kDa Observed band size:34 kDa)
Western Blot (WB)
(Anti-OTUB1 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse brain tissue lysate Lysates/proteins at 30 ug per Lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:31 kDa Observed band size:34 kDa)
Related Product Information for anti-OTUB1 antibody
Ubiquitin thioesterase OTUB1, also known as Deubiquitinating enzyme OTUB1, OTU domain-containing ubiquitin aldehyde-binding protein 1, Otubain-1, Ubiquitin-specific-processing protease OTUB1, OTUB1 and OTB1, is a cytoplasm protein that belongs to the peptidase C65 family. OTUB1 is a hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. OTUB1 is a regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. OTUB1 acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 of OTUB1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 of OTUB1 stabilizes RNF128 and promotes anergy. OTUB1 regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). OTUB1 mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. OTUB1 is also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin.
References
Balakirev M.Y., et al., 2003, EMBO Rep. 4:517-522. Soares L., et al., 2004, Nat. Immunol. 5:45-54. Stanisic V., et al., 2009, J. Biol. Chem. 284:16135-16145. Choudhary C., et al., 2009, Science 325:834-840. Edelmann M.J., et al., 2009, Biochem. J. 418:379-390.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,270 Da
NCBI Official Full Name
ubiquitin thioesterase OTUB1
NCBI Official Synonym Full Names
OTU domain, ubiquitin aldehyde binding 1
NCBI Official Symbol
Otub1
NCBI Official Synonym Symbols
AI850305
NCBI Protein Information
ubiquitin thioesterase OTUB1; otubain 1; otubain-1; deubiquitinating enzyme OTUB1; ubiquitin-specific-processing protease OTUB1; OTU domain-containing ubiquitin aldehyde-binding protein 1
UniProt Protein Name
Ubiquitin thioesterase OTUB1
Protein Family
UniProt Gene Name
Otub1
UniProt Entry Name
OTUB1_MOUSE
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Product Notes
The OTUB1 otub1 (Catalog #AAA8103491) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-OTUB1 Antibody, Rabbit Polyclonal reacts with Mouse Predicted to React with: Human, Rat, Bovine (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's OTUB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the OTUB1 otub1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OTUB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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