Rabbit RAB1A Polyclonal Antibody | anti-RAB1A antibody

Anti-RAB1A Antibody, Rabbit Polyclonal

Cat. Num. AAA8103706

• Gene Names: RAB1A; RAB1; YPT1
• Reactivity: Human; Predicted to React with: Pig (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: RAB1A; Polyclonal Antibody; Anti-RAB1A Antibody; Rabbit Polyclonal; RAB1A Antibody; Rabbit PAb; Antigen Affinity Purified; member RAS oncogene family; Anti-RAB1 Antibody; Anti-YPT1 Antibody; anti-RAB1A antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Pig (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human RAB1A
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-RAB1A antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:100-1:500
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human RAB1A
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human RAB1A, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-RAB1A rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:23 kDa Observed band size:23 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human RAB1A in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human RAB1A in human small intestine with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human RAB1A in mouse kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human RAB1A in mouse small intestine with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human RAB1A in rat kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human RAB1A in rat small intestine with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-RAB1A antibody

Rab1A is a newly identified mTORC1 activator that mediates an alternative amino acid (AA) signaling branch to Rag GTPases. Rab1a is a member of the Rab family of small GTPases with a well-characterized function in the regulation of vesicle trafficking from the endoplasmic reticulum to the Golgi apparatus and within Golgi compartments. RAB1A acts as an oncogene in various cancers, and emerging evidence has verified that RAB1A is a mTORC1 activator in hepatocellular and colorectal cancer. RAB1A acts as an oncogene by regulating cellular proliferation, growth, invasion, and metastasis via activation of the mTORC1 pathway in triple-negative breast cancer. RAB1A is an important small molecule in the mechanistic target of the rapamycin signaling pathway, which is one of the downstream signaling pathways of the epidermal growth factor receptor family.

Product Categories/Family for anti-RAB1A antibody

Autophagy Pathway

References

Xu H, et al. (2017) Inhibition of rab1a suppresses epithelial-mesenchymal transition and proliferation of triple-negative breast cancer cells. Oncol Rep 37 (3): 1619-1626.Xu B, et al. (2018) Significance and prognostic role of human epidermal growth factor receptor 2 and rab1a expression in gastric cancer. Oncol Lett 15 (4): 5185-5192.Wang C, et al. (2010) Regulation of integrin beta 1 recycling to lipid rafts by rab1a to promote cell migration. J Biol Chem 285 (38): 29398-29405.

NCBI and Uniprot Product Information

• NCBI GI #: 51338603
• NCBI GeneID: 5861
• UniProt Accession #: P62820; P11476; Q6FIE7; Q96N61; Q9Y3T2
• Molecular Weight: 205
• NCBI Official Full Name: Ras-related protein Rab-1A
• NCBI Official Synonym Full Names: RAB1A, member RAS oncogene family
• NCBI Official Symbol: RAB1A
• NCBI Official Synonym Symbols: RAB1; YPT1
• NCBI Protein Information: ras-related protein Rab-1A; YPT1-related protein; Rab GTPase YPT1 homolog; GTP binding protein Rab1a; RAB1, member RAS oncogene family
• UniProt Protein Name: Ras-related protein Rab-1A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB1A
• UniProt Synonym Gene Names: RAB1
• UniProt Entry Name: RAB1A_HUMAN

NCBI Description

This gene encodes a member of the Ras superfamily of GTPases. Members of the gene family cycle between inactive GDP-bound and active GTP-bound forms. This small GTPase controls vesicle traffic from the endoplasmic reticulum to the Golgi apparatus. Multiple alternatively spliced transcript variants have been identified for this gene which encode different protein isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

RAB1A: Probably required for transit of protein from the ER through Golgi compartment. Binds GTP and GDP and possesses intrinsic GTPase activity. Belongs to the small GTPase superfamily. Rab family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric; G protein, monomeric, Rab; G protein

Chromosomal Location of Human Ortholog: 2p14

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; early endosome; melanosome; cytosol

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding

Biological Process: ER to Golgi vesicle-mediated transport; cell migration; endocytosis; virus assembly; melanosome transport; vesicle-mediated transport; growth hormone secretion; intracellular protein transport; defense response to bacterium; vesicle transport along microtubule; autophagy; cilium biogenesis; mitotic cell cycle; Rab protein signal transduction; Golgi organization and biogenesis; autophagic vacuole formation