Rabbit ENO3 Polyclonal Antibody | anti-ENO3 antibody

Anti-ENO3 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103427

• Gene Names: Eno3; MSE; Eno-3
• Reactivity: Mouse; Predicted to React with: Human, Rat, Bovin, Pig (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: ENO3; Polyclonal Antibody; Anti-ENO3 Antibody; Rabbit Polyclonal; ENO3/beta-enolase Antibody; Rabbit PAb; Antigen Affinity Purified; enolase 3 (beta; muscle); Anti-Eno-3 Antibody; Anti-MSE Antibody; anti-ENO3 antibody

• Host: Rabbit
• Reactivity: Mouse; Predicted to React with: Human, Rat, Bovin, Pig (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse ENO3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ENO3 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 5-10uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse ENO3/beta-enolase
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse ENO3/beta-enolase, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ENO3 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Molt-4 Whole Cell Lysate Lane C: NIH-3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:47 kDa Observed band size:47 kDa)

Immunoprecipitation (IP)

(ENO3 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg NIH-3T3 Whole Cell Lysate Lane C:0.5 mg RAW246.7 Whole Cell Lysate 4 uL anti-ENO3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ENO3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa Observed band size :54 kDa)

Related Product Information for anti-ENO3 antibody

ENO3 is one of the three enolase isoenzymes found in mammals. As a homodimer, ENO3 is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in ENO3 gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for ENO3 gene that differ only in their 5' UTR. ENO3 may play a role in muscle development and regeneration. It appears to have a function in striated muscle development and regeneration.

References

Peshavaria M, et al. (1989) Structure of human muscle (beta) enolase mRNA and protein deduced from a genomic clone. Nucleic Acids Res. 17(21):8862. Cali L, et al. (1990) Nucleotide sequence of a cDNA encoding the human muscle-specific enolase (MSE). Nucleic Acids Res. 18(7):1893. Peshavaria M, et al. (1991) Molecular structure of the human muscle-specific enolase gene (ENO3). Biochem J. 275(2):427-33.

NCBI and Uniprot Product Information

• NCBI GI #: 209862931
• NCBI GeneID: 13808
• NCBI Accession #: NP_001129534.1
• NCBI GenBank Nucleotide #: NM_001136062.2
• UniProt Accession #: P21550
• Molecular Weight: 47,025 Da
• NCBI Official Full Name: beta-enolase
• NCBI Official Synonym Full Names: enolase 3, beta muscle
• NCBI Official Symbol: Eno3
• NCBI Official Synonym Symbols: MSE; Eno-3
• NCBI Protein Information: beta-enolase; muscle-specific enolase; skeletal muscle enolase; 2-phospho-D-glycerate hydro-lyase
• UniProt Protein Name: Beta-enolase
• Protein Family: Enolase
• UniProt Gene Name: Eno3
• UniProt Synonym Gene Names: Eno-3; MSE
• UniProt Entry Name: ENOB_MOUSE

NCBI Description

This gene encodes one of the three enolase isoenzymes found in vertebrates. Enolase is a dimeric enzyme that converts 2-phosphoglycerate to phosphoenolpyruvate as part of the glycolytic pathway. This isozyme is found in skeletal muscle where it is involved in muscle development and regeneration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Uniprot Description

ENO3: Appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13). A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen- beta particles are detected on ultrastructural analysis. Belongs to the enolase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Lyase

Cellular Component: extracellular space; phosphopyruvate hydratase complex; membrane; cytoplasm; plasma membrane

Molecular Function: protein homodimerization activity; protein heterodimerization activity; metal ion binding; lyase activity; magnesium ion binding; phosphopyruvate hydratase activity

Biological Process: glycolysis