Rabbit anti-Human, Mouse CLPX Polyclonal Antibody | anti-clpX antibody

CLPX, CT (CLPX, ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial)

Cat. Num. AAA643629

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: CLPX; Polyclonal Antibody; CT (CLPX; ATP-dependent Clp protease ATP-binding subunit clpX-like; mitochondrial); Anti -CLPX; anti-clpX antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-clpX antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: CLPX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-481 amino acids from the C-terminal region of human CLPX.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CLPX Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CLPX antibody detected the CLPX protein (arrow).)

Western Blot (WB)

(CLPX Antibody (C-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the CLPX antibody detected the CLPX protein (arrow).)

Immunohistochemistry (IHC)

(CLPX antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLPX antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CLPX Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-clpX antibody

ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP (By similarity).

Product Categories/Family for anti-clpX antibody

Antibodies; Abs to Mitochondrial Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 159030461
• UniProt Accession #: A8YMD8
• Molecular Weight: 49,078 Da
• NCBI Official Full Name: clpX
• UniProt Protein Name: ATP-dependent Clp protease ATP-binding subunit ClpX
• Protein Family: ATP-dependent Clp protease ATP-binding
• UniProt Gene Name: clpX
• UniProt Entry Name: A8YMD8_MICAE

Uniprot Description

Function: ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP

By similarity. HAMAP-Rule MF_00175 SAAS SAAS004487

Subunit structure: Heterodimer of ClpP and ClpX

By similarity. HAMAP-Rule MF_00175 SAAS SAAS004487

Sequence similarities: Belongs to the ClpX chaperone family. HAMAP-Rule MF_00175