Chromatin Immunoprecipitation (ChIP)
(Sox2 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from undifferentiated hESC cells and 7 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 10 million sequence tags were mapped to identify Sox2 binding sites. The image shows binding across a region of chromosome 7. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Rabbit anti-Human, Mouse Sox2 Polyclonal Antibody | anti-Sox2 antibody
Sox2 antibody (pAb)
ChIP: 10ul per ChIP
ChIP-Seq: 10ul each
ICC/IF: 1:200 dilution
WB: 1:500-1:1,000 dilution ChIP-Seq validation was performed's Epigenetics Services.
Chromatin Immunoprecipitation (ChIP)
(Sox2 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from undifferentiated hESC cells and 7 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 10 million sequence tags were mapped to identify Sox2 binding sites. The image shows binding across a region of chromosome 7. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Chromatin Immunoprecipitation (ChIP)
(Sox2 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from undifferentiated hESC cells and 7 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 10 million sequence tags were mapped to identify Sox2 binding sites. The image shows binding across a region of chromosome 7. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Immunofluorescence (IF)
(Sox2 antibody (pAb) tested by Immunofluorescence. Mouse embryonic stem cells (mESCs) grown on mouse embryonic fibroblast feeder cells (MEFs) were fixed with 4% paraformaldehyde for 10 minutes at room temperature. Cells were then permeabilized and blocked by incubating with Blocking Solution containing 5% serum/0.1% Triton X-100 in D-PBS for 2 hours at room temperature. Cells were then incubated with Sox2 antibody at 1:200 dilution overnight at 4 degree C, washed with D-PBS, and incubated for 2 hours at room temperature with goat anti-mouse Cy3 secondary antibody at 1:250 dilution. Cells were visualized using a Zeiss fluorescent microscope at 20X magnification. Images show that Sox2 antibody specifically stains mESC colonies and does not stain MEFs. Absence of Sox2 staining in a subset of cells within the colonies suggests differentiation. Scale bars, 100 ?m.)
Immunofluorescence (IF)
(Sox2 antibody (pAb) tested by Immunofluorescence. Mouse embryonic stem cells (mESCs) grown on mouse embryonic fibroblast feeder cells (MEFs) were fixed with 4% paraformaldehyde for 10 minutes at room temperature. Cells were then permeabilized and blocked by incubating with Blocking Solution containing 5% serum/0.1% Triton X-100 in D-PBS for 2 hours at room temperature. Cells were then incubated with Sox2 antibody at 1:200 dilution overnight at 4 degree C, washed with D-PBS, and incubated for 2 hours at room temperature with goat anti-mouse Cy3 secondary antibody at 1:250 dilution. Cells were visualized using a Zeiss fluorescent microscope at 20X magnification. Images show that Sox2 antibody specifically stains mESC colonies and does not stain MEFs. Absence of Sox2 staining in a subset of cells within the colonies suggests differentiation. Scale bars, 100 ?m.)
Western Blot (WB)
(Sox2 antibody (pAb) tested by Western blot. Nuclear extract of P19 cells (20 ug per lane) probed with Sox2 antibody (pAb) (1:500 dilution).)
Western Blot (WB)
(Sox2 antibody (pAb) tested by Western blot. Nuclear extract of P19 cells (20 ug per lane) probed with Sox2 antibody (pAb) (1:500 dilution).)
Background: Sox2 (SRY related HMG BOX gene 2) is a DNA binding transcription factor and a member of the SOX family of proteins. SOX proteins have an HMG box that binds DNA. Sox2 forms a complex with Oct-4 and controls the expression of a number of genes involved in embryonic. Sox2 is critical for early embryogenesis and for embryonic stem cell pluripotency and thus can serve as a stem cell marker. Overexpression of Sox2 (along with Oct-4, KLF4 and c-Myc) can transform mouse fibroblasts into a state resembling embryonic stem cells (ES cells), referred to as Induced Pluripotency. Defects in Sox2 are the cause of microphthalmia syndromic type 3.
NCBI and Uniprot Product Information
NCBI Description
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]
Research Articles on Sox2
Similar Products
Product Notes
The Sox2 sox2 (Catalog #AAA388603) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Sox2 antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Sox2 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). Applications Validated: ChIP: 10ul per ChIP ChIP-Seq: 10ul each ICC/IF: 1:200 dilution WB: 1:500-1:1,000 dilution ChIP-Seq validation was performed's Epigenetics Services. Researchers should empirically determine the suitability of the Sox2 sox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sox2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
