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SDS-Page

Adrenergic Receptor Beta Kinase 2 Recombinant Protein | ADRbK2 recombinant protein

Recombinant Adrenergic Receptor Beta Kinase 2 (ADRbK2)

Gene Names
Adrbk2; GRK3; Bark-2; Adrbk-2; AI851927; AW551196; 4833444A01Rik
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
>95%
Synonyms
Adrenergic Receptor Beta Kinase 2; Recombinant Adrenergic Receptor Beta Kinase 2 (ADRbK2); ADRbK2 recombinant protein
Ordering
For Research Use Only!
Host
Host: E Coli
Source: Prokaryotic expression
Purity/Purification
>95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
FSVHRIIGRG GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER IMLSLVSTGD CPFIVCMTYA FHTPDKLCFI LDLMNGGDLH YHLSQHGVFS EKEMRFYATE IILGLEHMHN RFVVYRDLKP ANILLDEHGH ARISDLGLAC DFSKKKPHAS VGTHGYMAPE VLQKGTAYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH EIDRMTLTVN VELPDTFSPE LKSLLEGLLQRDVSKRLGCH GGGSQEVKEH SFF
Sequence Length
688
Applicable Applications for ADRbK2 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Predicted Molecular Mass
33.5KDa
Accurate Molecular Mass (KD)
34kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
N-terminal His-Tag
Organism Species
Homo sapiens (Human)
Fragment
Phe191~Phe453
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
79,657 Da
NCBI Official Full Name
Adrenergic receptor kinase, beta 2
NCBI Official Synonym Full Names
adrenergic receptor kinase, beta 2
NCBI Official Symbol
Adrbk2
NCBI Official Synonym Symbols
GRK3; Bark-2; Adrbk-2; AI851927; AW551196; 4833444A01Rik
NCBI Protein Information
beta-adrenergic receptor kinase 2
UniProt Protein Name
Beta-adrenergic receptor kinase 2
UniProt Gene Name
Adrbk2
UniProt Synonym Gene Names
Beta-ARK-2
UniProt Entry Name
ARBK2_MOUSE

Uniprot Description

GRK3: Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Protein type: EC 2.7.11.15; Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; GRK family; BARK subfamily

Cellular Component: membrane; perinuclear region of cytoplasm; axon; dendritic spine; synapse; dendritic shaft; nucleus; Z disc; cytosol; cilium

Molecular Function: transferase activity; protein serine/threonine kinase activity; G-protein coupled receptor kinase activity; beta-adrenergic receptor kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; D1 dopamine receptor binding; ATP binding; protein kinase activity

Biological Process: intracellular protein transport; desensitization of G-protein coupled receptor protein signaling pathway; inositol phosphate metabolic process; receptor internalization; rhodopsin metabolic process; positive regulation of protein amino acid phosphorylation; signal transduction; protein amino acid phosphorylation; phosphorylation

Research Articles on ADRbK2

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Product Notes

The ADRbK2 adrbk2 (Catalog #AAA2031355) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Adrenergic Receptor Beta Kinase 2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the ADRbK2 adrbk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
FSVHRIIGR G GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER IMLSLVSTGD CPFIVCMTYA FHTPDKLCFI LDLMNGGDLH YHLSQHGVFS EKEMRFYATE IILGLEHMHN RFVVYRDLKP ANILLDEHGH ARISDLGLAC DFSKKKPHAS VGTHGYMAPE VLQKGTAYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH EIDRMTLTVN VELPDTFSPE LKSLLEGLLQ RDVSKRLGCH GGGSQEVKEH SFF. It is sometimes possible for the material contained within the vial of "Adrenergic Receptor Beta Kinase 2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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