Rabbit anti-Human, Mouse CLPX Polyclonal Antibody | anti-CLPX antibody

CLPX, CT (CLPX, ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial) (AP)

Cat. Num. AAA6286921

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CLPX; Polyclonal Antibody; CT (CLPX; ATP-dependent Clp protease ATP-binding subunit clpX-like; mitochondrial) (AP); mitochondrial; anti-CLPX antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CLPX antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CLPX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 452-481 amino acids from the C-terminal region of human CLPX.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CLPX Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CLPX antibody detected the CLPX protein (arrow).)

Western Blot (WB)

(CLPX Antibody (C-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the CLPX antibody detected the CLPX protein (arrow).)

Immunohistochemistry (IHC)

(CLPX antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLPX antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CLPX Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CLPX antibody

ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP (By similarity).

Product Categories/Family for anti-CLPX antibody

Antibodies; Mitochondrial Proteins

References

Martin, A., et al. Mol. Cell 29(4):441-450(2008). Bogenhagen, D.F., et al. J. Biol. Chem. 283(6):3665-3675(2008). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Kang, S.G., et al. J. Biol. Chem. 280(42):35424-35432(2005). Kang, S.G., et al. J. Biol. Chem. 277(23):21095-21102(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 7242140
• NCBI GeneID: 10845
• NCBI Accession #: NP_006651.2
• NCBI GenBank Nucleotide #: NM_006660.3
• UniProt Accession #: O76031; Q9H4D9; A1L428; A8K8F1; B9EGI8
• Molecular Weight: 69,224 Da
• NCBI Official Full Name: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial
• NCBI Official Synonym Full Names: caseinolytic mitochondrial matrix peptidase chaperone subunit
• NCBI Official Symbol: CLPX
• NCBI Protein Information: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial; ClpX caseinolytic protease X homolog; ClpX caseinolytic peptidase X homolog; energy-dependent regulator of proteolysis
• UniProt Protein Name: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial
• Protein Family: ATP-dependent Clp protease ATP-binding
• UniProt Gene Name: CLPX
• UniProt Entry Name: CLPX_HUMAN