Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CLIC4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 2s.)
Rabbit CLIC4 Polyclonal Antibody | anti-CLIC4 antibody
CLIC4 Polyclonal Antibody
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CLIC4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 2s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CLIC4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 2s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat brain using CLIC4 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat brain using CLIC4 antibody at dilution of 1:100 (40x lens).)
NCBI and Uniprot Product Information
NCBI Description
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq, Jul 2008]
Uniprot Description
CLIC4: Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell- surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical- basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Belongs to the chloride channel CLIC family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 1p36.11
Cellular Component: centrosome; cell surface; nuclear matrix; microvillus; cytoplasmic vesicle membrane; mitochondrion; intercellular junction; cytosol; actin cytoskeleton; microtubule cytoskeleton; apical part of cell; perinuclear region of cytoplasm; cytoplasm; plasma membrane; intracellular; midbody
Molecular Function: chloride channel activity; protein binding; voltage-gated ion channel activity
Biological Process: keratinocyte differentiation; vacuolar acidification; fertilization; branching morphogenesis of a tube; apoptosis; multicellular organism growth; establishment and/or maintenance of apical/basal cell polarity; chloride transport; angiogenesis; endothelial cell morphogenesis; cell differentiation; negative regulation of cell migration; regulation of cytoskeleton organization and biogenesis
Research Articles on CLIC4
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Product Notes
The CLIC4 clic4 (Catalog #AAA129971) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CLIC4 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CLIC4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:100. Researchers should empirically determine the suitability of the CLIC4 clic4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLIC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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