Mouse Sphingosine 1 Phosphate Receptor 5 (S1PR5) ELISA Kit | S1PR5 elisa kit

Mouse Sphingosine 1 Phosphate Receptor 5 (S1PR5) ELISA Kit

Cat. Num. AAA2705581

• Gene Names: S1PR5; EDG8; S1P5; Edg-8; SPPR-1; SPPR-2
• Reactivity: Mouse
• Synonyms: Sphingosine 1 Phosphate Receptor 5 (S1PR5); Mouse Sphingosine 1 Phosphate Receptor 5 (S1PR5) ELISA Kit; EDG8; S1P5; S1PR5; SPPR-1; SPPR-2; Endothelial Differentiation; Sphingolipid G-Protein-Coupled Receptor; 8; Sphingosine 1-phosphate receptor Edg-8; S1PR5 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sphingosine 1 Phosphate Receptor 5 (S1PR5).; No significant cross-reactivity or interference between Sphingosine 1 Phosphate Receptor 5 (S1PR5) and analogues was observed.
• Sequence Length: 398

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sphingosine 1 Phosphate Receptor 5 (S1PR5) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sphingosine 1 Phosphate Receptor 5 (S1PR5) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S1PR5 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Receptor 5 (S1PR5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Receptor 5 (S1PR5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine 1 Phosphate Receptor 5 (S1PR5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine 1 Phosphate Receptor 5 (S1PR5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 261278306
• NCBI GeneID: 53637
• NCBI Accession #: NP_001159687.1
• NCBI GenBank Nucleotide #: NM_001166215.1
• UniProt Accession #: Q9H228; Q6NW11
• Molecular Weight: 32,279 Da
• NCBI Official Full Name: sphingosine 1-phosphate receptor 5
• NCBI Official Synonym Full Names: sphingosine-1-phosphate receptor 5
• NCBI Official Symbol: S1PR5
• NCBI Official Synonym Symbols: EDG8; S1P5; Edg-8; SPPR-1; SPPR-2
• NCBI Protein Information: sphingosine 1-phosphate receptor 5
• UniProt Protein Name: Sphingosine 1-phosphate receptor 5
• Protein Family: Sphingosine 1-phosphate receptor
• UniProt Gene Name: S1PR5
• UniProt Synonym Gene Names: EDG8; S1P receptor 5; S1P5; S1P receptor Edg-8

NCBI Description

The lysosphingolipid sphingosine 1-phosphate (S1P) regulates cell proliferation, apoptosis, motility, and neurite retraction. Its actions may be both intracellular as a second messenger and extracellular as a receptor ligand. S1P and the structurally related lysolipid mediator lysophosphatidic acid (LPA) signal cells through a set of G protein-coupled receptors known as EDG receptors. Some EDG receptors (e.g., EDG1; MIM 601974) are S1P receptors; others (e.g., EDG2; MIM 602282) are LPA receptors.[supplied by OMIM, Mar 2008]

Uniprot Description

EDG8: Receptor for the lysosphingolipid sphingosine 1- phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/0)alpha and G(12) subclass of heteromeric G-proteins. May play a regulatory role in the transformation of radial glial cells into astrocytes and may affect proliferative activity of these cells. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: integral component of membrane; plasma membrane

Biological Process: regulation of neuron differentiation