PRPS1 recombinant protein

PRPS1, 1-318aa, Human, His tag, E Coli

Cat. Num. AAA203513

• Gene Names: PRPS1; ARTS; DFN2; PRSI; CMTX5; DFNX1; PRS-I; PPRibP
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: PRPS1; 1-318aa; Human; His tag; E Coli; Ribose-phosphate pyrophosphokinase 1; ARTS; CMTX5; PPRibP; PRSI; DFN2; DFNX1; Ribose-phosphate pyrophosphokinase 1 EC 2.7.6.1; KIAA0967; Phosphoribosyl pyrophosphate synthetase I; PRS I; PRS-I; Ribose phosphate pyrophosphokinase I; ; PRPS1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 0.1 M NaCl, and 20% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MPNIKIFSGS SHQDLSQKIA DRLGLELGKV VTKKFSNQET CVEIGESVRG EDVYIVQSGC GEINDNLMEL LIMINACKIA SASRVTAVIP CFPYARQDKK DKSRAPISAK LVANMLSVAG ADHIITMDLH ASQIQGFFDI PVDNLYAEPA VLKWIRENIS EWRNCTIVSP DAGGAKRVTS IADRLNVDFA LIHKERKKAN EVDRMVLVGD VKDRVAILVD DMADTCGTIC HAADKLLSAG ATRVYAILTH GIFSGPAISR INNACFEAVV VTNTIPQEDK MKHCSKIQVI DISMILAEAI RRTHNGESVS YLFSHVPL
• Sequence Length: 318

• Applicable Applications for PRPS1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for PRPS1 recombinant protein

PRPS1, also known as ribose-phosphate pyrophosphokinase 1, is ubiquitously expressed in human tissues. This enzyme catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for purine metabolism and nucleotide biosynthesis. Recombinant human PRPS1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PRPS1 recombinant protein

Enzymes & Proteases

References

Liu H., et al. (2009) Biochem Biophys Res Commun. 379 (4):1120-5. ; Li S., et al. (2007) Biochem J. 401 (1):39-47.;

NCBI and Uniprot Product Information

• NCBI GI #: 4506127
• NCBI GeneID: 5631
• NCBI Accession #: NP_002755
• NCBI GenBank Nucleotide #: NM_002764.3
• UniProt Accession #: P60891; P09329; B1ALA8; B2R6T7; B4DNL6; D3DUX6
• Molecular Weight: 36.9 kDa (338aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: ribose-phosphate pyrophosphokinase 1 isoform 1
• NCBI Official Synonym Full Names: phosphoribosyl pyrophosphate synthetase 1
• NCBI Official Symbol: PRPS1
• NCBI Official Synonym Symbols: ARTS; DFN2; PRSI; CMTX5; DFNX1; PRS-I; PPRibP
• NCBI Protein Information: ribose-phosphate pyrophosphokinase 1
• UniProt Protein Name: Ribose-phosphate pyrophosphokinase 1
• UniProt Gene Name: PRPS1
• UniProt Synonym Gene Names: PRS-I

NCBI Description

This gene encodes an enzyme that catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for purine metabolism and nucleotide biosynthesis. Defects in this gene are a cause of phosphoribosylpyrophosphate synthetase superactivity, Charcot-Marie-Tooth disease X-linked recessive type 5 and Arts Syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

Catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis.