Rabbit CLIC4 Polyclonal Antibody | anti-CLIC4 antibody

CLIC4, NT (Chloride Intracellular Channel Protein 4, Intracellular Chloride Ion Channel Protein p64H1, CLIC4)

Cat. Num. AAA646989

• Gene Names: CLIC4; H1; huH1; p64H1; CLIC4L; MTCLIC
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography
• Synonyms: CLIC4; Polyclonal Antibody; NT (Chloride Intracellular Channel Protein 4; Intracellular Chloride Ion Channel Protein p64H1; CLIC4); Anti -CLIC4; anti-CLIC4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CLIC4. Species Crossreactivity: Mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography
• Form/Format: Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-CLIC4 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunohistochemistry.; Dilution: ELISA: 1:40,000; Western Blot: 1:500-1:1000; Immunohistochemistry (Paraffin): 10ug/ml
• Immunogen: Synthetic peptide corresponding to human CLIC4 at N-Terminal.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of extracts from HT-29 cells, using CLIC4. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-CLIC4 antibody

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).

Product Categories/Family for anti-CLIC4 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 7330335
• NCBI GeneID: 25932
• NCBI Accession #: NP_039234.1
• NCBI GenBank Nucleotide #: NM_013943.2
• UniProt Accession #: Q9Y696; Q9UFW9; Q9UQJ6
• Molecular Weight: 28,772 Da
• NCBI Official Full Name: chloride intracellular channel protein 4
• NCBI Official Synonym Full Names: chloride intracellular channel 4
• NCBI Official Symbol: CLIC4
• NCBI Official Synonym Symbols: H1; huH1; p64H1; CLIC4L; MTCLIC
• NCBI Protein Information: chloride intracellular channel protein 4; chloride intracellular channel 4 like; intracellular chloride ion channel protein p64H1
• UniProt Protein Name: Chloride intracellular channel protein 4
• Protein Family: Chloride intracellular channel protein
• UniProt Gene Name: CLIC4
• UniProt Entry Name: CLIC4_HUMAN

NCBI Description

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Ref.7 Ref.9 Ref.10 Ref.13 Ref.15 Ref.19

Subunit structure: Component of a multimeric complex consisting of several cytoskeletal proteins, including actin, ezrin, alpha-actinin, gelsolin, IQGAP1 and CLIC5A. Binds directly to brain dynamin I in a complex containing actin, tubulin and 14-3-3 isoforms. Monomer. Interacts with HRH3. Interacts with AKAP9. Ref.6 Ref.8 Ref.13 Ref.19

Subcellular location: Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cytoplasmic vesicle membrane; Single-pass membrane protein

Probable. Nucleus matrix. Cell membrane; Single-pass membrane protein

Probable. Mitochondrion. Cell junction. Note: Colocalized with AKAP9 at the centrosome and midbody. Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles. Ref.9 Ref.11 Ref.12 Ref.13 Ref.19

Tissue specificity: Detected in epithelial cells from colon, esophagus and kidney (at protein level). Expression is prominent in heart, kidney, placenta and skeletal muscle. Ref.6 Ref.11 Ref.12

Induction: Up-regulated by calcium ions in differentiating keratinocytes. Up-regulated in myofibroblasts. Ref.7 Ref.12

Domain: Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion. Ref.20

Sequence similarities: Belongs to the chloride channel CLIC family.Contains 1 GST C-terminal domain.