Rabbit anti-Human Claudin 12 Polyclonal Antibody | anti-CLDN12 antibody

Rabbit anti Claudin 12 Polyclonal Antibody

Cat. Num. AAA460028

• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Epitope Affinity Purification
• Synonyms: Claudin 12; Polyclonal Antibody; Rabbit anti Claudin 12 Polyclonal Antibody; CLDN12; anti-CLDN12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody reacts human, mouse and rat. The other species are not tested.
• Purity/Purification: Purified by Epitope Affinity Purification
• Form/Format: Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Sequence Length: 244

• Applicable Applications for anti-CLDN12 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: 0.01-0.1 ug/ml; Flow Cytometry: 5-10 ug/ml; Immunohistochemistry: 2-10 ug/ml; Immunoprecipitation: 2-5 ug/ml; Western Blot: 0.1-1 ug/ml
• Preparation: A synthetic peptide from C-terminus of human claudin 12 protein. This sequence is identical to human, rat, mouse and bovine.
• Preparation and Storage: The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Related Product Information for anti-CLDN12 antibody

The claudin superfamily consists of many structurally related proteins in humans. These proteins, which include claudin 1 through 18, are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Claudins, which consist of four trans-membrane domains and two extracellular loops, make up tight junction strands. Claudin 12 is 244 aa residues. It is expressed in the inner ear and bladder epithelium, and it is over-expressed in colorectal carcinomas.

References

Heiskala, M., Peterson, P.A. and Yang, Y. The roles of claudin superfamily proteins in paracellular transport. Traffic 2 (2), 93-98 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 51094918
• NCBI GeneID: 9069
• Molecular Weight: 27,110 Da
• NCBI Official Full Name: claudin 12
• NCBI Official Synonym Full Names: claudin 12
• NCBI Official Symbol: CLDN12
• NCBI Protein Information: claudin-12
• UniProt Protein Name: Claudin-12
• Protein Family: Claudin
• UniProt Gene Name: CLDN12

NCBI Description

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is expressed in the inner ear and bladder epithelium, and it is over-expressed in colorectal carcinomas. This protein and claudin 2 are critical for vitamin D-dependent Ca2+ absorption between enterocytes. Multiple alternatively spliced transcript variants encoding the same protein have been found.[provided by RefSeq, Sep 2011]

Uniprot Description

Claudin-12: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Belongs to the claudin family.

Protein type: Cell adhesion; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q21.13

Cellular Component: plasma membrane; tight junction

Molecular Function: identical protein binding; protein binding

Biological Process: calcium-independent cell-cell adhesion