Annexin VI/ANXA6 Active Protein | ANXA6 active protein

Annexin VI/ANXA6 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120710

• Gene Names: ANXA6; ANX6; CBP68
• Purity: >97% as determined by SDS-PAGE
• Synonyms: Annexin VI/ANXA6; Annexin VI/ANXA6 Protein; Human; Recombinant (His Tag); Human ANXA6/Annexin A6/Annexin6 Protein (His Tag); ANX6 Protein; CBP68 Protein; annexin A6; ANXA6 active protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp673

• Species: Human
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human ANXA6/Annexin A6/Annexin6 (P08133)(Met 1-Asp 673) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its ability to bind human CD21-His in functional Elisa.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ANXA6 active protein

Background: Annexin A6, also known as ANXA6 or ANXAI¥, belongs to a family of Ca²⁺-dependent membrane and phospholipid binding proteins. Members of this family have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 6 is phosphorylated in vivo associated with cell growth. Annexin 6 was not phosphorylated in quiescent cells, but was phosphorylated on serine and to a lesser extent threonine, several hours following cell stimulation. Experiment has revealed the presence of annexin A6 on the cell surface of variety cells as putative receptors and/or binding proteins for chondroitin sulfate proteoglycans, helping cells to bind with this extracellular matrix glycosaminoglycan chondroitin sulfate which is related to the cell-substratum adhesion. A post-tranlational modification other than direct protein phosphorylation may influence the activity of annexin6 and provide evidence linking cell growth with regulation of annexin 6 function.

References

Takagi H, et al. (2002) Annexin 6 is a putative cell surface receptor for chondroitin sulfate chains. J Cell Sci. 115 (16): 3309-18.Moss SE, et al. (1992) A growth-dependent post-translational modification of annexin VI. Biochim Biophys Acta. 1160 (1): 120-6.Song G, et al. (1998) Altered cardiac annexin mRNA and protein levels in the left ventricle of patients with end-stage heart failure. J Mol Cell Cardio. 30 (3): 443-51.

NCBI and Uniprot Product Information

• NCBI GI #: 71773329
• NCBI GeneID: 309
• NCBI Accession #: NP_001146.2
• NCBI GenBank Nucleotide #: NM_001155.4
• UniProt Accession #: P08133; Q6ZT79; B7Z8A7; D3DQH4; E9PGK1
• Molecular Weight: 75,873 Da
• NCBI Official Full Name: annexin A6 isoform 1
• NCBI Official Synonym Full Names: annexin A6
• NCBI Official Symbol: ANXA6
• NCBI Official Synonym Symbols: ANX6; CBP68
• NCBI Protein Information: annexin A6; p68; p70; CPB-II; annexin-6; calelectrin; lipocortin VI; calphobindin II; calphobindin-II; chromobindin-20; annexin VI (p68); 67 kDa calelectrin; calcium-binding protein p68
• UniProt Protein Name: Annexin A6
• Protein Family: Annexin
• UniProt Gene Name: ANXA6
• UniProt Synonym Gene Names: ANX6; CPB-II
• UniProt Entry Name: ANXA6_HUMAN

NCBI Description

Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2010]

Uniprot Description

ANXA6: a calcium/phospholipid-binding protein that may associate with CD21. May regulate the release of Ca(2+) from intracellular stores. Phosphorylated in response to growth factor stimulation. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Motility/polarity/chemotaxis; Calcium-binding; Lipid-binding

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: focal adhesion; membrane; lysosomal membrane; late endosome membrane; perinuclear region of cytoplasm; melanosome

Molecular Function: protein binding; protein homodimerization activity; GTP binding; calcium-dependent phospholipid binding; cholesterol binding; calcium ion binding; lipid binding; calcium-dependent protein binding; ligand-gated ion channel activity

Biological Process: regulation of muscle contraction; calcium ion transport; protein homooligomerization