Western Blot (WB)
(Western blot analysis of CKIP1 in human lung tissue lysate with CKIP1 antibody at (A) 1 and (B) 2 ug/mL.)
Rabbit CKIP1 Polyclonal Antibody | anti-CKIP1 antibody
CKIP1 Antibody
Gene Names
PLEKHO1; JBP; CKIP1; OC120; CKIP-1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity chromatography purified via peptide column
Synonyms
CKIP1; Polyclonal Antibody; CKIP1 Antibody; Casein kinase-2-interacting protein 1; pleckstrin homology domain containing; family O member 1; PLECKHO1; anti-CKIP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Affinity chromatography purified via peptide column
Form/Format
Supplied in PBS containing 0.02% sodium azide.
Sequence Length
409
Applicable Applications for anti-CKIP1 antibody
ELISA (EIA), Western Blot (WB)
Immunogen Type
Peptide
Immunogen Description
Raised against an 18 amino acid peptide from near the carboxy terminus of human CKIP1.
Target Name
CKIP1
Preparation and Storage
Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of CKIP1 in human lung tissue lysate with CKIP1 antibody at (A) 1 and (B) 2 ug/mL.)
Western Blot (WB)
(Western blot analysis of CKIP1 in human lung tissue lysate with CKIP1 antibody at (A) 1 and (B) 2 ug/mL.)
Related Product Information for anti-CKIP1 antibody
CKIP1 was identified through a yeast-two hybrid screening as a protein that would interact with only one of the two catalytic subunits of the casein kinase 2 complex. CKIP1 is a pleckstrin homology domain-containing protein localized within the nucleus and at the plasma membrane that interacts with CK2alpha but not CK2alpha', and is thought to play a role in targeting CK2alpha to a particular cellular location. CKIP1 has been implicated in muscle differentiation and the regulation of cell morphology and actin cytoskeleton. CKIP1 can also interact with other proteins such as ATM, an upstream kinase of p53, and recruit the nuclear ATM to the plasma membrane, suggesting CKIP1 may regulate ATM function through re-localizing ATM. CKIP1 can also form a complex with the kinase AKT, leading to a down-regulation of PI3K/AKT signaling and suppression of tumor growth in vivo.
Product Categories/Family for anti-CKIP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,330 Da
NCBI Official Full Name
pleckstrin homology domain-containing family O member 1 isoform a
NCBI Official Synonym Full Names
pleckstrin homology domain containing, family O member 1
NCBI Official Symbol
PLEKHO1
NCBI Official Synonym Symbols
JBP; CKIP1; OC120; CKIP-1
NCBI Protein Information
pleckstrin homology domain-containing family O member 1
UniProt Protein Name
Pleckstrin homology domain-containing family O member 1
UniProt Gene Name
PLEKHO1
UniProt Synonym Gene Names
CKIP1; OC120; PH domain-containing family O member 1; JBP; CK2-interacting protein 1; CKIP-1
UniProt Entry Name
PKHO1_HUMAN
Uniprot Description
PLEKHO1: Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT- mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold; Tumor suppressor
Chromosomal Location of Human Ortholog: 1q21.2
Cellular Component: cytoplasm; plasma membrane; nucleus
Molecular Function: protein binding
Research Articles on CKIP1
Similar Products
Product Notes
The CKIP1 plekho1 (Catalog #AAA9412634) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CKIP1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CKIP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the CKIP1 plekho1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CKIP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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