Mouse anti-Human PLEKHO1 Polyclonal Antibody | anti-PLEKHO1 antibody

PLEKHO1 (Pleckstrin Homology Domain-containing Family O Member 1, PH Domain-containing Family O Member 1, C-Jun-binding Protein, JBP, Casein Kinase 2-interacting Protein 1, CK2-interacting Protein 1, CKIP-1, Osteoclast Maturation-associated Gene 120 Prote

Cat. Num. AAA6003892

• Gene Names: PLEKHO1; OC120; CKIP-1; RP11-458I7.3
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PLEKHO1; Polyclonal Antibody; PLEKHO1 (Pleckstrin Homology Domain-containing Family O Member 1; PH Domain-containing Family O Member 1; C-Jun-binding Protein; JBP; Casein Kinase 2-interacting Protein 1; CK2-interacting Protein 1; CKIP-1; Osteoclast Maturation-associated Gene 120 Prote; Anti -PLEKHO1 (Pleckstrin Homology Domain-containing Family O Member 1; anti-PLEKHO1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PLEKHO1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MMKKNNSAKRGPQDGNQQPAPPEKVGWVRKFCGKGIFREIWKNRYVVLKGDQLYISEKEVKDEKNIQEVFDLSDYEKCEELRKSKSRSKKNHSKFTLAHSKQPGNTAPNLIFLAVSPEEKESWINALNSAITRAKNRILDEVTVEEDSYLAHPTRDRAKIQHSRRPPTRGHLMAVASTSTSDGMLTLDLIQEEDPSPEEPTSCAESFRVDLDKSVAQLAGSRRRADSDRIQPSADRASSLSRPWEKTDKGATYTPQAPKKLTPTEKGRCASLEEILSQRDAASARTLQLRAEEPPTPALPNPGQLSRIQDLVARKLEETQELLAEVQGLGDGKRKAKDPPRSPPDSESEQLLLETERLLGEASSNWSQAKRVLQEVRELRDLYRQMDLQTPDSHLRQTTPHSQYRKSLM

• Applicable Applications for anti-PLEKHO1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence: 10ug/ml
• Immunogen: Full length human PLEKHO1, aa1-409 (NP_057358.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PLEKHO1 polyclonal antibody. Western Blot analysis of PLEKHO1 expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of PLEKHO1 expression in transfected 293T cell line by PLEKHO1 polyclonal antibody. Lane 1: PLEKHO1 transfected lysate (44.99kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of purified antibody to PLEKHO1 on HeLa cell. [antibody concentration 10ug/ml].)

Related Product Information for anti-PLEKHO1 antibody

CKIP-1 plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). This protein may function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). It appears to target ATM to the plasma membrane and appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation.

Product Categories/Family for anti-PLEKHO1 antibody

Antibodies; Abs to Pleckstrin Homology Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 33871771
• NCBI GeneID: 51177
• UniProt Accession #: Q53GL0; Q336K5; Q8IZ51; Q9NRV3; Q9UL48
• Molecular Weight: 46,237 Da
• NCBI Official Full Name: PLEKHO1 protein, partial
• NCBI Official Synonym Full Names: pleckstrin homology domain containing, family O member 1
• NCBI Official Symbol: PLEKHO1
• NCBI Official Synonym Symbols: OC120; CKIP-1; RP11-458I7.3
• NCBI Protein Information: pleckstrin homology domain-containing family O member 1; JBP; OC120; 2810052M02Rik; C-Jun-binding protein; CK2-interacting protein 1; casein kinase 2-interacting protein 1; PH domain-containing family O member 1; CK2 interacting protein 1; HQ0024c protein; osteoclast maturation-associated gene 120 protein
• UniProt Protein Name: Pleckstrin homology domain-containing family O member 1
• Protein Family: Pleckstrin homology domain-containing family
• UniProt Gene Name: PLEKHO1
• UniProt Synonym Gene Names: CKIP1; PH domain-containing family O member 1; JBP; CK2-interacting protein 1; CKIP-1
• UniProt Entry Name: PKHO1_HUMAN

Uniprot Description

PLEKHO1: Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT- mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding