Human Pleckstrin homology domain-containing family O member 1 ELISA Kit | PLEKHO1 elisa kit

Human Pleckstrin homology domain-containing family O member 1 ELISA Kit

Cat. Num. AAA763629

• Gene Names: PLEKHO1; JBP; CKIP1; OC120; CKIP-1
• Reactivity: Human
• Synonyms: Pleckstrin homology domain-containing family O member 1; Human Pleckstrin homology domain-containing family O member 1 ELISA Kit; PLEKHO1/Pleckstrin homology domain-containing family O member 1/PH domain-containing family O member 1/Osteoclast maturation-associated gene 120 protein/C-Jun-binding protein/JBP/Casein kinase 2-interacting protein 1/CK2-interacting protein 1/CKIP-1; PLEKHO1 elisa kit

• Reactivity: Human
• Sequence Length: 409

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 0.625-40ng/ml
• Sensitivity: 0.375ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PLEKHO1 elisa kit

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLEKHO1. During the reaction,Human PLEKHO1 in the sample or standard competes with a fixed amount of biotin-labeled Human PLEKHO1 for sites on a pre-coated Monoclonal antibody specific to Human PLEKHO1. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human PLEKHO1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 20149626
• NCBI GeneID: 51177
• NCBI Accession #: NP_057358.2
• NCBI GenBank Nucleotide #: NM_016274.5
• Molecular Weight: 42,330 Da
• NCBI Official Full Name: pleckstrin homology domain-containing family O member 1 isoform a
• NCBI Official Synonym Full Names: pleckstrin homology domain containing O1
• NCBI Official Symbol: PLEKHO1
• NCBI Official Synonym Symbols: JBP; CKIP1; OC120; CKIP-1
• NCBI Protein Information: pleckstrin homology domain-containing family O member 1
• UniProt Protein Name: Pleckstrin homology domain-containing family O member 1
• Protein Family: Pleckstrin homology domain-containing family
• UniProt Gene Name: PLEKHO1
• UniProt Synonym Gene Names: CKIP1; OC120; PH domain-containing family O member 1; JBP; CK2-interacting protein 1; CKIP-1
• UniProt Entry Name: PKHO1_HUMAN

Uniprot Description

PLEKHO1: Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT- mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Tumor suppressor

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: myoblast fusion; myoblast migration; regulation of cell shape