Rabbit YTHDC1 Polyclonal Antibody | anti-YTHDC1 antibody

YTHDC1 Rabbit pAb

Cat. Num. AAA4757661

• Gene Names: YTHDC1; YT521; YT521-B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Affinity purified
• Synonyms: YTHDC1; Polyclonal Antibody; YTHDC1 Rabbit pAb; YTH domain-containing protein 1; anti-YTHDC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Applicable Applications for anti-YTHDC1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:100
• Immunogen: Recombinant protein of human YTHDC1 (NP_001026902.1).
• Conjugation: Unconjugated
• Molecular Function: Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs (PubMed:26318451, PubMed:26876937, PubMed:25242552). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:26318451, PubMed:25242552). Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10 (PubMed:26876937). Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed:26876937). In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (PubMed:26876937). May also regulate alternative splice site selection (PubMed:20167602).
• Modification: Unmodified
• Cell Position: Nucleus
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot - YTHDC1 Polyclonal Antibody.Western blot analysis of extracts of various cell lines, using YTHDC1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.)

Immunofluorescence (IF)

(Immunofluorescence - YTHDC1 Polyclonal Antibody. Immunofluorescence analysis of A-549 cells using YTHDC1 antibody . Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-YTHDC1 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 72534750
• NCBI GeneID: 91746
• NCBI Accession #: NP_001026902.1
• NCBI GenBank Nucleotide #: NM_001031732.2
• UniProt Accession #: Q96MU7; Q4W5Q3; Q7Z622; Q8TF35
• Molecular Weight: 82,692 Da
• NCBI Official Full Name: YTH domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: YTH domain containing 1
• NCBI Official Symbol: YTHDC1
• NCBI Official Synonym Symbols: YT521; YT521-B
• NCBI Protein Information: YTH domain-containing protein 1; splicing factor YT521-B; putative splicing factor YT521
• UniProt Protein Name: YTH domain-containing protein 1
• Protein Family: YTH domain-containing protein
• UniProt Gene Name: YTHDC1
• UniProt Synonym Gene Names: KIAA1966; YT521; YT521-B
• UniProt Entry Name: YTDC1_HUMAN