Western Blot (WB)
(Western blot analysis of GATA1 expression in K562 cell lysate.)
Rabbit anti-Human GATA1 Polyclonal Antibody | anti-GATA1 antibody
Anti-GATA1 Rabbit pAb
Gene Names
GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Affinity Chromatography
Synonyms
GATA1; Polyclonal Antibody; Anti-GATA1 Rabbit pAb; Erythroid transcription factor; Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding protein; ERYF1; GF1; anti-GATA1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
GATA1 Antibody detects endogenous levels of total GATA1
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
413
Applicable Applications for anti-GATA1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
Immunogen
A synthesized peptide derived from human GATA1
Subcellular Location
Nucleus
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot analysis of GATA1 expression in K562 cell lysate.)
Western Blot (WB)
(Western blot analysis of GATA1 expression in K562 cell lysate.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse spleen, using GATA1 Antibody.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse spleen, using GATA1 Antibody.)
Related Product Information for anti-GATA1 antibody
GATA-1 is the founding member of the GATA family and is required for erythroid and megakaryocytic cell development. Mutations in GATA-1 have been linked to many human diseases, including acute megakaryoblastic leukemia in Down syndrome children (DS-AMKL), X-linked thrombocytopenia, and gray platelet syndrome.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43kDa
NCBI Official Full Name
erythroid transcription factor
NCBI Official Synonym Full Names
GATA binding protein 1
NCBI Official Symbol
GATA1
NCBI Official Synonym Symbols
GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1
NCBI Protein Information
erythroid transcription factor
UniProt Protein Name
Erythroid transcription factor
Protein Family
UniProt Gene Name
GATA1
UniProt Synonym Gene Names
ERYF1; GF1; GATA-1; GF-1
NCBI Description
This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq, Jul 2008]
Uniprot Description
Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781).
Research Articles on GATA1
Similar Products
Product Notes
The GATA1 gata1 (Catalog #AAA4750880) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GATA1 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GATA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC/IF: 1:50-1:200 IP: 1:50. Researchers should empirically determine the suitability of the GATA1 gata1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GATA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
