Goat CIZ1 Polyclonal Antibody | anti-CIZ1 antibody

Goat anti-CIZ1 Antibody

Cat. Num. AAA423340

• Gene Names: CIZ1; NP94; LSFR1; ZNF356
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: CIZ1; Polyclonal Antibody; Goat anti-CIZ1 Antibody; CDKN1A interacting zinc finger protein 1; LSFR1; NP94; ZNF356; CDKN1A-interacting zinc finger protein 1; cip1-interacting zinc finger protein; nuclear protein NP94; zinc finger protein 356; CIZ1 antibody; CDKN1A interacting zinc finger protein 1 antibody; LSFR1 antibody; NP94 antibody; ZNF356 antibody; CDKN1A-interacting zinc finger protein 1 antibody; cip1-interacting zinc finger protein antibody; nuclear protein NP94 antibody; zinc finger protein 356 antibody; anti-CIZ1 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: QPNTQDKTPSKVTAR
• Sequence Length: 898

• Applicable Applications for anti-CIZ1 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Western Blot: Approx 90kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 91.2kDa according to NP_001124490.1). Recommended concentration: 0.3-1ug/ml.
• Immunogen: Peptide with sequence C-QPNTQDKTPSKVTAR, from the C Terminus of the protein sequence according to NP_036259.2; NP_001124487.1; NP_001124489.1; NP_001124490.1; NP_001244904.1; NP_001244905.1.
• Epitope: C Terminus
• Note: This antibody is expected to recognize all reported isoforms (NP_036259.2; NP_001124487.1; NP_001124489.1; NP_001124490.1; NP_001244904.1; NP_001244905.1). Reported variants represent identical protein: NP_036259.2, NP_001124488.1
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of HeLa nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 42544179
• NCBI GeneID: 25792
• NCBI Accession #: NP_036259.2; NP_001124487.1; NP_001124489.1; NP_001124490.1; NP_001244904.1; NP_001244905.1
• NCBI GenBank Nucleotide #: NM_012127.2
• Molecular Weight: 88,520 Da
• NCBI Official Full Name: cip1-interacting zinc finger protein isoform 1
• NCBI Official Synonym Full Names: CDKN1A interacting zinc finger protein 1
• NCBI Official Symbol: CIZ1
• NCBI Official Synonym Symbols: NP94; LSFR1; ZNF356
• NCBI Protein Information: cip1-interacting zinc finger protein
• UniProt Protein Name: Cip1-interacting zinc finger protein
• Protein Family: Cip1-interacting zinc finger protein
• UniProt Gene Name: CIZ1
• UniProt Synonym Gene Names: LSFR1; NP94; ZNF356
• UniProt Entry Name: CIZ1_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger DNA binding protein that interacts with CIP1, part of a complex with cyclin E. The encoded protein may regulate the cellular localization of CIP1. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

CIZ1: May regulate the subcellular localization of CIP/WAF1. Defects in CIZ1 may be a cause of adult onset primary cervical dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Cervical dystonia or spasmodic torticollis, the most common form of focal dystonia, is characterized by involuntary contractions of the neck muscles, which produce abnormal posturing of the head upon the trunk. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: nucleus

Molecular Function: nucleic acid binding; protein binding; zinc ion binding