Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Goat Semaphorin 5A Polyclonal Antibody | anti-SEMA5A antibody
Goat anti-Semaphorin 5A Antibody
Immunohistochemistry: In paraffin embedded Human Kidney shows membanous and vesicular staining in PCT cells. Recommended concentration, 4-6ug/ml.
Western Blot: Preliminary experiments gave an approx 90kDa in Mouse Brain lysates and approx 25kDa band in Human Brain (Cerebellum), Colon, Heart and Kidney lysates after 0. 03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 121kDa according to NP_003957.1. The 90kDa and 25kDa band were successfully blocked by incubation with the immunizing peptide.
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.[provided by RefSeq, Jan 2010]
Uniprot Description
SEMA5A: a single-pass type I membrane protein that may act as positive axonal guidance cues.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 5p15.2
Cellular Component: integral to membrane; membrane; plasma membrane
Molecular Function: chemorepellent activity; heparan sulfate proteoglycan binding; semaphorin receptor binding; syndecan binding
Biological Process: axonal fasciculation; blood vessel endothelial cell proliferation during sprouting angiogenesis; cell adhesion; cell-cell signaling; diencephalon development; negative chemotaxis; negative regulation of axon extension involved in axon guidance; negative regulation of cell adhesion; nervous system development; neural crest cell migration; positive chemotaxis; positive regulation of actin filament depolymerization; positive regulation of angiogenesis; positive regulation of axon extension involved in axon guidance; positive regulation of cell migration; positive regulation of endothelial cell proliferation; positive regulation of protein kinase B signaling cascade
Research Articles on SEMA5A
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Product Notes
The SEMA5A sema5a (Catalog #AAA421475) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Semaphorin 5A Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Semaphorin 5A can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Kidney shows membanous and vesicular staining in PCT cells. Recommended concentration, 4-6ug/ml. Western Blot: Preliminary experiments gave an approx 90kDa in Mouse Brain lysates and approx 25kDa band in Human Brain (Cerebellum), Colon, Heart and Kidney lysates after 0. 03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 121kDa according to NP_003957.1. The 90kDa and 25kDa band were successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the SEMA5A sema5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DEATKKACYS KGKS. It is sometimes possible for the material contained within the vial of "Semaphorin 5A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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