Mouse Cip1-interacting zinc finger protein (CIZ1) ELISA Kit | CIZ1 elisa kit

Mouse Cip1-interacting zinc finger protein (CIZ1) ELISA Kit

Cat. Num. AAA7232836

• Gene Names: CIZ1; NP94; LSFR1; ZNF356
• Reactivity: Mouse
• Synonyms: Cip1-interacting zinc finger protein (CIZ1); Mouse Cip1-interacting zinc finger protein (CIZ1) ELISA Kit; CIZ1 elisa kit

• Reactivity: Mouse

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CIZ1 elisa kit

Intended Uses: This CIZ1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanCIZ1. This ELISA kit for research use only!

Principle of the Assay: CIZ1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CIZ1 antibody and an CIZ1-HRP conjugate. The assay sample and buffer are incubated together with CIZ1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CIZ1 concentration since CIZ1 from samples and CIZ1-HRP conjugate compete for the anti-CIZ1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CIZ1 from the sample, fewer sites are left to bind CIZ1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CIZ1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CIZ1 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 42544179
• NCBI GeneID: 25792
• NCBI Accession #: NP_036259.2
• NCBI GenBank Nucleotide #: NM_012127.2
• Molecular Weight: 88,520 Da
• NCBI Official Full Name: cip1-interacting zinc finger protein isoform 1
• NCBI Official Synonym Full Names: CDKN1A interacting zinc finger protein 1
• NCBI Official Symbol: CIZ1
• NCBI Official Synonym Symbols: NP94; LSFR1; ZNF356
• NCBI Protein Information: cip1-interacting zinc finger protein; nuclear protein NP94; zinc finger protein 356
• UniProt Protein Name: Cip1-interacting zinc finger protein
• Protein Family: Cip1-interacting zinc finger protein
• UniProt Gene Name: CIZ1
• UniProt Synonym Gene Names: LSFR1; NP94; ZNF356
• UniProt Entry Name: CIZ1_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger DNA binding protein that interacts with CIP1, part of a complex with cyclin E. The encoded protein may regulate the cellular localization of CIP1. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

CIZ1: May regulate the subcellular localization of CIP/WAF1. Defects in CIZ1 may be a cause of adult onset primary cervical dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Cervical dystonia or spasmodic torticollis, the most common form of focal dystonia, is characterized by involuntary contractions of the neck muscles, which produce abnormal posturing of the head upon the trunk. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: nucleus

Molecular Function: protein binding; nucleic acid binding; zinc ion binding