Western Blot (WB)
(Mouse Chuk Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Chuk antibody detected the Chuk protein (arrow).)
Rabbit anti-Mouse Chuk Polyclonal Antibody | anti-Chuk antibody
Chuk, CT (Chuk, Ikka, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, Nuclear factor NF-kappa-B inhibitor kinase alpha) (Biotin)
Gene Names
Chuk; IKK1; Ikka; Chuk1; Fbx24; IKBKA; Fbxo24; NFKBIKA; AI256658
Reactivity
Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Chuk; Polyclonal Antibody; CT (Chuk; Ikka; Inhibitor of nuclear factor kappa-B kinase subunit alpha; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; Nuclear factor NF-kappa-B inhibitor kinase alpha) (Biotin); Nuclear factor NF-kappa-B inhibitor kinase alpha; anti-Chuk antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-Chuk antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
Mouse Chuk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 550-578 amino acids from the C-terminal region of Mouse Chuk.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Mouse Chuk Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Chuk antibody detected the Chuk protein (arrow).)
Western Blot (WB)
(Mouse Chuk Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Chuk antibody detected the Chuk protein (arrow).)
Immunohistochemistry (IHC)
(Mouse Chuk Antibody (C-term) (AP14612b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Chuk Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Mouse Chuk Antibody (C-term) (AP14612b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Chuk Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-Chuk antibody
Chuk acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.
Product Categories/Family for anti-Chuk antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,729 Da
NCBI Official Full Name
inhibitor of nuclear factor kappa-B kinase subunit alpha isoform X1
NCBI Official Synonym Full Names
conserved helix-loop-helix ubiquitous kinase
NCBI Official Symbol
Chuk
NCBI Official Synonym Symbols
IKK1; Ikka; Chuk1; Fbx24; IKBKA; Fbxo24; NFKBIKA; AI256658
NCBI Protein Information
inhibitor of nuclear factor kappa-B kinase subunit alpha; IKK-A; IKK alpha; I kappa B kinase 1; I-kappa-B kinase 1; IkappaB kinase alpha; I-kappa-B kinase alpha; nuclear factor NF-kappa-B inhibitor kinase alpha
UniProt Protein Name
Inhibitor of nuclear factor kappa-B kinase subunit alpha
Protein Family
UniProt Gene Name
Chuk
UniProt Synonym Gene Names
Ikka; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; IKK1; NFKBIKA
UniProt Entry Name
IKKA_MOUSE
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Product Notes
The Chuk chuk (Catalog #AAA6286043) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Chuk, CT (Chuk, Ikka, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, Nuclear factor NF-kappa-B inhibitor kinase alpha) (Biotin) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Chuk can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Chuk chuk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chuk, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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