Rabbit anti-Human TRPM7 Polyclonal Antibody | anti-TRPM7 antibody

TRPM7, NT (Transient Receptor Potential Cation Channel Subfamily M Member 7, Long Transient Receptor Potential Channel 7, LTrpC-7, Channel-kinase 1, CHAK1, LTRPC7)

Cat. Num. AAA648520

• Gene Names: TRPM7; CHAK; CHAK1; ALSPDC; LTRPC7; TRP-PLIK
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: TRPM7; Polyclonal Antibody; NT (Transient Receptor Potential Cation Channel Subfamily M Member 7; Long Transient Receptor Potential Channel 7; LTrpC-7; Channel-kinase 1; CHAK1; LTRPC7); Anti -TRPM7; anti-TRPM7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human TRPM7.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-TRPM7 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:1000; Western Blot: 1:100-1:500; Immunohistochemistry (Paraffin): 10ug/ml
• Immunogen: Synthetic peptide corresponding to TRPM7 from human CHAK1 at N-Terminal conjugated to KLH.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(TRPM7(10ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human thyroid, followed by biotinylated goat, rabbit IgG, alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-TRPM7 antibody

Channel kinase 1 is an Alpha-kinase with a novel catalytic domain that is fused to a calcium-permeant ion channel. The kinase domain of TRPM7 directly associates with the C2 domain of phospholipase C.

Product Categories/Family for anti-TRPM7 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 148612863
• NCBI GeneID: 54822
• NCBI Accession #: NP_060142.3
• NCBI GenBank Nucleotide #: NM_017672.4
• UniProt Accession #: Q96QT4; Q6ZMF5; Q86VJ4; Q8NBW2; Q9BXB2; Q9NXQ2
• Molecular Weight: 212,697 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily M member 7
• NCBI Official Synonym Full Names: transient receptor potential cation channel, subfamily M, member 7
• NCBI Official Symbol: TRPM7
• NCBI Official Synonym Symbols: CHAK; CHAK1; ALSPDC; LTRPC7; TRP-PLIK
• NCBI Protein Information: transient receptor potential cation channel subfamily M member 7; LTrpC-7; channel-kinase 1; LTRPC ion channel family member 7; long transient receptor potential channel 7; transient receptor potential-phospholipase C-interacting kinase
• UniProt Protein Name: Transient receptor potential cation channel subfamily M member 7
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: TRPM7
• UniProt Synonym Gene Names: CHAK1; LTRPC7; LTrpC-7; LTrpC7
• UniProt Entry Name: TRPM7_HUMAN

NCBI Description

The protein encoded by this gene is both an ion channel and a serine/threonine protein kinase. The kinase activity is essential for the ion channel function, which serves to increase intracellular calcium levels and to help regulate magnesium ion homeostasis. Defects in this gene are a cause of amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam.[provided by RefSeq, May 2010]

Uniprot Description

Function: Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1). Ref.6 Ref.7

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Binds 1 zinc ion per subunit

By similarity.

Subunit structure: Homodimer. Interacts with PLCB1

By similarity. Forms heterodimers with TRPM6. Ref.8

Subcellular location: Membrane; Multi-pass membrane protein

Probable.

Post-translational modification: Autophosphorylated

By similarity.

Involvement in disease: Amyotrophic lateral sclerosis-parkinsonism/dementia complex 1 (ALS-PDC1) [MIM:105500]: A neurodegenerative disorder characterized by chronic, progressive and uniformly fatal amyotrophic lateral sclerosis and parkinsonism-dementia. Both diseases are known to occur in the same kindred, the same sibship and even the same individual.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.

Sequence similarities: In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.In the N-terminal section; belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM7 sub-subfamily. [View classification]Contains 1 alpha-type protein kinase domain.

Sequence caution: The sequence BAC11462.1 differs from that shown. Reason: Erroneous initiation. The sequence BAD18773.1 differs from that shown. Reason: Erroneous initiation.