Rabbit anti-Mouse Chuk Polyclonal Antibody | anti-Chuk antibody

Mouse Chuk Antibody (C-term)

Cat. Num. AAA9205016

• Gene Names: Chuk; IKK1; Ikka; Chuk1; Fbx24; IKBKA; Fbxo24; NFKBIKA; AI256658
• Reactivity: Mouse
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Chuk; Polyclonal Antibody; Mouse Chuk Antibody (C-term); Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; Ikka; anti-Chuk antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Mouse Chuk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 551-578 amino acids from the C-terminal region of mouse Chuk.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.45 (varies by lot)
• Sequence Positions: 551-578
• Sequence Length: 745

• Applicable Applications for anti-Chuk antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: MOUSE
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Mouse Chuk Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Chuk antibody detected the Chuk protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Chuk Antibody (C-term) (MBS9205016)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Chuk Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Chuk antibody

Chuk acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.

Product Categories/Family for anti-Chuk antibody

Cardiovascular; Cell Biology; Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 14285502
• NCBI GeneID: 12675
• UniProt Accession #: Q60680; Q80VU2; Q9D2X3
• Molecular Weight: 84729
• NCBI Official Full Name: Inhibitor of nuclear factor kappa-B kinase subunit alpha
• NCBI Official Synonym Full Names: conserved helix-loop-helix ubiquitous kinase
• NCBI Official Symbol: Chuk
• NCBI Official Synonym Symbols: IKK1; Ikka; Chuk1; Fbx24; IKBKA; Fbxo24; NFKBIKA; AI256658
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit alpha
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit alpha
• Protein Family: Inhibitor of nuclear factor kappa-B kinase
• UniProt Gene Name: Chuk
• UniProt Synonym Gene Names: Ikka; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; IKK1; NFKBIKA
• UniProt Entry Name: IKKA_MOUSE

Uniprot Description

IKKA: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B, leading to their dissociation from NF-kappa-B and ultimately to their degradation. Phosphorylated and activated downstream of growth factor receptors, IL1R, and TNFR by NAK, IRAK and NIK, respectively. Preferentially found as a heterodimer with IKK-beta but also as an homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP. Heterodimers form the active complex. The tripartite complex can also bind to MAP3K14/NIK, MEKK1, IKAP and IKB-alpha-p65-p50 complex. Inhibitors are under development to treat arthritis, inflammation, and apoptotic aspects of cancer. Aspirin (sodium salicylate) selectively binds IKK and reduces inflammation. Misexpression and inhibitors show involvement in insulin sensitization in obese rodents. Mutations in IKK-gamma are found in several immune deficiencies: hyper IgM syndrome, incontinentia pigmenti [embryonic lethal in males, defects in skin, hair, teeth in females] and hypohidrotic ectodermal dysplasia; recurrent infections and defects in teeth, hair, and sweat glands]. Inhibitors: CHS 828, NBD peptide, BMS-345541.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.10; Kinase, protein; Protein kinase, Other; Other group; IKK family

Cellular Component: nucleoplasm; internal side of plasma membrane; intracellular membrane-bound organelle; cytoplasm; IkappaB kinase complex; cytosol; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: lactation; positive regulation of I-kappaB kinase/NF-kappaB cascade; mammary gland epithelial cell proliferation; response to lipopolysaccharide; osteoclast differentiation; protein amino acid phosphorylation; Rho protein signal transduction; odontogenesis of dentine-containing teeth; response to organic substance; morphogenesis of an epithelial sheet; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; I-kappaB phosphorylation; phosphorylation