Western Blot (WB)
(Western Blot analysis of NCK1 on different lysates using anti-NCK1 antibody at 1/500 dilution. Positive control:Lane 1: Hela Lane 1: NIH-3T3Lane 2: Rat kidney tissueLane 3: Mouse testis tissue)
Rabbit NCK1 Polyclonal Antibody | anti-NCK1 antibody
NCK1 Polyclonal Antibody
ICC: 1:50-1:200
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
IP: 1:10-1:50
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of NCK1 on different lysates using anti-NCK1 antibody at 1/500 dilution. Positive control:Lane 1: Hela Lane 1: NIH-3T3Lane 2: Rat kidney tissueLane 3: Mouse testis tissue)
Western Blot (WB)
(Western Blot analysis of NCK1 on different lysates using anti-NCK1 antibody at 1/500 dilution. Positive control:Lane 1: Hela Lane 1: NIH-3T3Lane 2: Rat kidney tissueLane 3: Mouse testis tissue)
Immunocytochemistry (ICC)
(ICC staining NCK1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining NCK1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Jun 2010]
Uniprot Description
NCK1: adapter protein containing Src homology 2 and 3 (SH2 and SH3) domains. Transduces signals from tyrosine-phosphorylated growth factor receptors to their cellular substrates.
Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 3q21
Cellular Component: endoplasmic reticulum; cytoplasm; plasma membrane; ribosome; protein phosphatase type 1 complex; intercellular junction; vesicle membrane; cytosol; nucleus
Molecular Function: protein binding, bridging; protein domain specific binding; protein binding; receptor signaling complex scaffold activity; protein kinase inhibitor activity; SH3/SH2 adaptor activity; cytoskeletal adaptor activity; receptor tyrosine kinase binding; receptor binding
Biological Process: lamellipodium biogenesis; axon guidance; positive regulation of translation in response to stress; T cell activation; negative regulation of peptidyl-serine phosphorylation; signal complex assembly; actin filament organization; response to other organism; T cell receptor signaling pathway; substrate-bound cell migration, cell extension; positive regulation of actin filament polymerization; negative regulation of protein kinase activity; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of T cell proliferation; vascular endothelial growth factor receptor signaling pathway
Research Articles on NCK1
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Product Notes
The NCK1 nck1 (Catalog #AAA3013101) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCK1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NCK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:500-1:2,000 ICC: 1:50-1:200 IHC: 1:50-1:200 FC/FACS: 1:50-1:100 IP: 1:10-1:50. Researchers should empirically determine the suitability of the NCK1 nck1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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