Immunohistochemistry (IHC)
Rabbit anti-Mouse, Rat Choline Acetyltransferase/Chat Polyclonal Antibody | anti-Chat antibody
Anti-Choline Acetyltransferase/Chat Antibody
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Immunogen affinity purified.
Synonyms
Choline Acetyltransferase/Chat; Polyclonal Antibody; Anti-Choline Acetyltransferase/Chat Antibody; Choline O-acetyltransferase (EC:2.3.1.6); CHOACTase; ChAT; Choline acetylase; Chat; anti-Chat antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
640
Applicable Applications for anti-Chat antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml (Rat)
IHC-P (Embedded Section): 0.5-1ug/ml (Mouse, Rat)
Direct ELISA: 0.1-0.5ug/ml (Rat)
Tested Species: In-house tested species with positive results.
IHC-P (Embedded Section): 0.5-1ug/ml (Mouse, Rat)
Direct ELISA: 0.1-0.5ug/ml (Rat)
Tested Species: In-house tested species with positive results.
Immunogen
E Coli-derived rat Choline Acetyltransferase/Chat recombinant protein (Position: E19-D612).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Western Blot (WB)
(Figure 1. Western blot analysis of Chat using anti-Chat antibody.)
Western Blot (WB)
(Figure 1. Western blot analysis of Chat using anti-Chat antibody.)
Related Product Information for anti-Chat antibody
Description: Rabbit IgG polyclonal antibody for Choline Acetyltransferase/Chat detection. Tested with WB, IHC-P, Direct ELISA in Mouse; Rat.
Background: Choline acetyltransferase (commonly abbreviated as ChAT, but sometimes CAT) is a transferase enzyme responsible for the synthesis of the neurotransmitter acetylcholine. In humans, the choline acetyltransferase enzyme is encoded by the CHAT gene. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.
Background: Choline acetyltransferase (commonly abbreviated as ChAT, but sometimes CAT) is a transferase enzyme responsible for the synthesis of the neurotransmitter acetylcholine. In humans, the choline acetyltransferase enzyme is encoded by the CHAT gene. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.
References
1. Cohen-Haguenauer, O., Brice, A., Berrard, S., Van Cong, N., Mallet, J., Frezal, J. Localization of the choline acetyltransferase (CHAT) gene to human chromosome 10. Genomics 6: 374-378, 1990.
2. Harold, D., Peirce, T., Moskvina, V., Myers, A., Jones, S., Hollingworth, P., Moore, P., Lovestone, S., Powell, J., Foy, C., Archer, N., Walter, S., and 11 others. Sequence variation in the CHAT locus shows no association with late-onset Alzheimer's disease. Hum. Genet. 113: 258-267, 2003.
3. Kraner, S, Laufenberg, I., Strassburg, H. M., Sieb, J. P., Steinlein, O. K. Congenital myasthenic syndrome with episodic apnea in patients homozygous for a CHAT missense mutation. Arch. Neurol. 60: 761-763, 2003.
2. Harold, D., Peirce, T., Moskvina, V., Myers, A., Jones, S., Hollingworth, P., Moore, P., Lovestone, S., Powell, J., Foy, C., Archer, N., Walter, S., and 11 others. Sequence variation in the CHAT locus shows no association with late-onset Alzheimer's disease. Hum. Genet. 113: 258-267, 2003.
3. Kraner, S, Laufenberg, I., Strassburg, H. M., Sieb, J. P., Steinlein, O. K. Congenital myasthenic syndrome with episodic apnea in patients homozygous for a CHAT missense mutation. Arch. Neurol. 60: 761-763, 2003.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Choline O-acetyltransferase
NCBI Official Synonym Full Names
choline O-acetyltransferase
NCBI Official Symbol
Chat
NCBI Protein Information
choline O-acetyltransferase
UniProt Protein Name
Choline O-acetyltransferase
Protein Family
UniProt Gene Name
Chat
UniProt Synonym Gene Names
CHOACTase; ChAT; Choline acetylase
UniProt Entry Name
CLAT_RAT
Uniprot Description
CHAT: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses
Protein type: Lipid Metabolism - glycerophospholipid; EC 2.3.1.6; Acetyltransferase
Cellular Component: neuron projection; cell soma; mitochondrion; axon; cytoplasm
Molecular Function: choline binding; choline O-acetyltransferase activity
Biological Process: response to drug; muscle development; rhythmic excitation; metabolic process; antral ovarian follicle growth; adult walking behavior; memory; neuron differentiation; synaptic transmission; rhythmic behavior; response to ethanol; acetylcholine biosynthetic process; response to hypoxia; dendrite development; neuromuscular synaptic transmission; establishment of synaptic specificity at neuromuscular junction; response to nutrient; neurotransmitter biosynthetic process
Research Articles on Chat
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Product Notes
The Chat chat (Catalog #AAA1752970) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Choline Acetyltransferase/Chat Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Choline Acetyltransferase/Chat can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA). WB: 0.25-0.5ug/ml (Rat) IHC-P (Embedded Section): 0.5-1ug/ml (Mouse, Rat) Direct ELISA: 0.1-0.5ug/ml (Rat) Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the Chat chat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Choline Acetyltransferase/Chat, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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