Western Blot (WB)
(Western Blot (WB) analysis of CHAC1 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:PC12 whole cell lysate)
Rabbit CHAC1 Polyclonal Antibody | anti-CHAC1 antibody
CHAC1 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
CHAC1; Polyclonal Antibody; CHAC1 Polyclonal Antibody; Cation transport regulator-like protein 1; Blocks Notch protein; Botch; BOTCH; anti-CHAC1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CHAC1 polyclonal antibody detects endogenous levels of CHAC1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
219
Applicable Applications for anti-CHAC1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human CHAC1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of CHAC1 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:PC12 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of CHAC1 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:PC12 whole cell lysate)
Related Product Information for anti-CHAC1 antibody
CHAC1 (ChaC, cation transport regulator homolog 1 (E. coli)) is a 264 amino acid cytoplasmic protein that belongs to the ChaC family. Induced by chemical activators of the unfolded protein response (UPR) pathway, including DTT, tunicamycin and thapsigargin, CHAC1 is a pro-apoptotic member of this pathway and may mediate the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. Overexpression of CHAC1 may lead to an increase in apoptosis, while suppression of CHAC1 results in subdued apoptosis. Existing as two alternatively spliced isoforms, the gene encoding CHAC1 maps to human chromosome 15q15.1. Encoding more than 700 genes, chromosome 15 is made up of approximately 106 million base pairs and comprises about 3% of the human genome. Angelman, Prader-Willi and Marfan syndromes are associated with loss of function or deletion of genes that are encoded by chromosome 15.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 24-28kDa
NCBI Official Full Name
glutathione-specific gamma-glutamylcyclotransferase 1 isoform b
UniProt Protein Name
Cation transport regulator-like protein 1
Protein Family
UniProt Gene Name
CHAC1
UniProt Synonym Gene Names
BOTCH; Botch
UniProt Entry Name
CHAC1_HUMAN
Uniprot Description
CHAC1: Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos. May also act as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. Belongs to the chaC family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 15q15.1
Cellular Component: trans-Golgi network; cytosol
Molecular Function: protein binding; transferase activity, transferring acyl groups; Notch binding
Biological Process: Notch signaling pathway; neurogenesis; metabolic process; negative regulation of Notch signaling pathway; response to unfolded protein
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Product Notes
The CHAC1 chac1 (Catalog #AAA3006404) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHAC1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CHAC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the CHAC1 chac1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHAC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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