Typical Testing Data/Standard Curve (for reference only)
Rat Flotillin-1 (FLOT1) ELISA Kit | FLOT1 elisa kit
Rat Flotillin-1 (FLOT1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: FLOT-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FLOT-1 antibody and an FLOT-1-HRP conjugate. The assay sample and buffer are incubated together with FLOT-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FLOT-1 concentration since FLOT-1 from samples and FLOT-1-HRP conjugate compete for the anti-FLOT-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by FLOT-1 from the sample, fewer sites are left to bind FLOT-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FLOT-1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
FLOT1: May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2. Interacts with ECM29. Belongs to the band 7/mec-2 family. Flotillin subfamily.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: signalosome; focal adhesion; membrane; lysosomal membrane; integral to membrane; melanosome; microtubule organizing center; caveola; flotillin complex; sarcolemma; endosome; lipid raft
Molecular Function: ionotropic glutamate receptor binding; protein binding; protease binding
Biological Process: extracellular matrix disassembly; axonogenesis; regulation of Rho protein signal transduction
Research Articles on FLOT1
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Product Notes
The Rat FLOT1 flot1 (Catalog #AAA7220479) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7220479 ELISA Kit recognizes Rat FLOT1. It is sometimes possible for the material contained within the vial of "Flotillin-1 (FLOT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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