Rabbit anti-Human DNA Damage Inducible Transcript 3 (DDIT3) Polyclonal Antibody | anti-DDIT3 antibody

Polyclonal Antibody to DNA Damage Inducible Transcript 3 (DDIT3)

Cat. Num. AAA2003530

• Gene Names: DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; C/EBPzeta
• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: DNA Damage Inducible Transcript 3 (DDIT3); Polyclonal Antibody; Polyclonal Antibody to DNA Damage Inducible Transcript 3 (DDIT3); anti-DDIT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF-MAAESLPFSF GTLSSWELEA WYEDLQEVLS SDENGGTYVS PPGNEEEESK IFTTLDPASL AWLTEEEPEP AEVTSTSQSP HSPDSSQSSL AQEEEEEDQG RTRKRKQSGH SPARAGKQRM KEKEQENERK VAQLAEENER LKQEIERLTR EVEATRRALI DRMVNLHQA

• Applicable Applications for anti-DDIT3 antibody: Western Blot (WB); Immunohistochemistry (IHC) - Formalin/Paraffin; Immunocytochemistry (ICC); Immunoprecipitation (IP)
• Application Notes: Western Blotting (WB): 0.5-2ug/mL; Immunohistochemistry (IHC): 5-20ug/mL; Immunocytochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user.
• Organism Species: Homo sapiens (Human)
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Cross Reactivity: Human
• Immunogen: Recombinant DDIT3 (Met1~Ala169 (Accession # P35638)) expressed in E.coli
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 4ºC for frequent use.; Aliquot and store at -20ºC for 24 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant DDIT3, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Stomach Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Tissue.Primary Ab: 20ug/ml Rabbit Anti-Human DDIT3 AntibodySecond Ab: 2 ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

NCBI and Uniprot Product Information

• NCBI GI #: 304282225
• NCBI GeneID: 1649
• NCBI Accession #: NP_001181982.1
• NCBI GenBank Nucleotide #: NM_001195053.1
• UniProt Accession #: P35638; F8VS99
• NCBI Official Full Name: DNA damage-inducible transcript 3 protein isoform 1
• NCBI Official Synonym Full Names: DNA damage inducible transcript 3
• NCBI Official Symbol: DDIT3
• NCBI Official Synonym Symbols: CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; C/EBPzeta
• NCBI Protein Information: DNA damage-inducible transcript 3 protein
• UniProt Protein Name: DNA damage-inducible transcript 3 protein
• Protein Family: DNA damage-inducible transcript 3 protein
• UniProt Gene Name: DDIT3
• UniProt Synonym Gene Names: CHOP; CHOP10; GADD153; DDIT-3; CHOP; CHOP-10

NCBI Description

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]

Uniprot Description

DDIT3: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform.

Protein type: Autophagy; DNA-binding; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: CHOP-ATF3 complex; CHOP-ATF4 complex; CHOP-C/EBP complex; cytosol; late endosome; nucleoplasm; nucleus; transcription factor AP-1 complex

Molecular Function: cAMP response element binding protein binding; DNA binding; DNA binding transcription factor activity; leucine zipper domain binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription corepressor activity; transcription factor binding; transcription regulatory region DNA binding

Biological Process: ATF6-mediated unfolded protein response; blood vessel maturation; cell cycle arrest; cell redox homeostasis; cellular response to DNA damage stimulus; ER overload response; establishment of protein localization to mitochondrion; inhibition of CREB transcription factor; intrinsic apoptotic signaling pathway in response to nitrosative stress; mRNA transcription from RNA polymerase II promoter; negative regulation of canonical Wnt signaling pathway; negative regulation of determination of dorsal identity; negative regulation of fat cell differentiation; negative regulation of myoblast differentiation; negative regulation of protein kinase B signaling cascade; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; PERK-mediated unfolded protein response; positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway; positive regulation of interleukin-8 production; positive regulation of neuron apoptotic process; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress; positive regulation of transcription, DNA-templated; proteasomal ubiquitin-dependent protein catabolic process; protein oligomerization; regulation of DNA-templated transcription in response to stress; regulation of transcription involved in anterior/posterior axis specification; regulation of transcription, DNA-templated; release of sequestered calcium ion into cytosol; response to starvation; response to unfolded protein; Wnt signaling pathway

Disease: Myxoid Liposarcoma