Western Blot (WB)
(CENP-B antibody (pAb) tested by Immunoprecipitation. 10 ul of CENP-B antibody was used to immunoprecipitate CENP-B from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the CENP-B antibody at a dilution of 1:500.)
Rabbit anti-Human CENP-B Polyclonal Antibody | anti-CENP-B antibody
CENP-B antibody (pAb)
IP: 10ul per IP
WB: 1:500-1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot.
Western Blot (WB)
(CENP-B antibody (pAb) tested by Immunoprecipitation. 10 ul of CENP-B antibody was used to immunoprecipitate CENP-B from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the CENP-B antibody at a dilution of 1:500.)
Western Blot (WB)
(CENP-B antibody (pAb) tested by Immunoprecipitation. 10 ul of CENP-B antibody was used to immunoprecipitate CENP-B from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the CENP-B antibody at a dilution of 1:500.)
Western Blot (WB)
(CENP-B antibody (pAb) tested by Western blot. CENP-B detection by Western blot. The analysis was performed using 30 ug HeLa nuclear cell extract and CENP-B at a 1:500 dilution.)
Western Blot (WB)
(CENP-B antibody (pAb) tested by Western blot. CENP-B detection by Western blot. The analysis was performed using 30 ug HeLa nuclear cell extract and CENP-B at a 1:500 dilution.)
Background: CENP-B (Centromere protein B) is a protein associated with centromeric DNA and crucial to the formation of the special chromatin structure on the a-satellite DNA repeats of the human centromere. CENP-B specifically binds a 17 bp sequence which may function as a cis-element for centromere-specific nucleosome assembly. CENP-B plays a role in the organization of centromere satellite DNA into a higher order structure, directing centromere formation and kinetochore assembly in mammalian chromosomes.
NCBI and Uniprot Product Information
NCBI Description
This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. [provided by RefSeq, Jul 2008]
Uniprot Description
CENPB: Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 20p13
Cellular Component: chromosome; nucleus; chromosome, pericentric region
Molecular Function: centromeric DNA binding; sequence-specific DNA binding; satellite DNA binding; chromatin binding
Biological Process: regulation of transcription, DNA-dependent
Research Articles on CENP-B
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Product Notes
The CENP-B cenpb (Catalog #AAA388842) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CENP-B antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CENP-B can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB). Applications Validated: IP: 10ul per IP WB: 1:500-1:2,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. Researchers should empirically determine the suitability of the CENP-B cenpb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CENP-B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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