Rabbit anti-Human ATAD2 Polyclonal Antibody | anti-ATAD2 antibody

ATAD2 antibody (pAb)

Cat. Num. AAA388867

• Gene Names: ATAD2; ANCCA; CT137; PRO2000
• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Purity: Affinity Purified
• Synonyms: ATAD2; Polyclonal Antibody; ATAD2 antibody (pAb); ANCCA; CT137; PRO2000; anti-ATAD2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Concentration: 0.46ug/ul (varies by lot)

• Applicable Applications for anti-ATAD2 antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Applications Validated:; IP: 5-10ul per IP; WB: 1:500-1:2,000 dilution; Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
• Immunogen: This antibody was raised against a peptide within the C-terminal region of human ATAD2.
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

Western Blot (WB)

(ATAD2 antibody (pAb) tested by Immunoprecipitation. 10 ul of ATAD2 antibody was used to immunoprecipitate ATAD2 from 250 ug of Jurkat nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the ATAD2 antibody at a dilution of 1:1,000.)

Western Blot (WB)

(ATAD2 antibody (pAb) tested by Western blot. ATAD2 detection by Western blot. The analysis was performed using 40 ug Jurkat nuclear cell extract and CENP-B at a 1:1,000 dilution.)

Related Product Information for anti-ATAD2 antibody

Short Description: Our ATAD2 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Human samples.

Background: ATAD2 (ATPase family AAA domain-containing protein 2) may be a transcriptional coactivator of the nuclear receptor ESR1 required to induce the expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. May play a role in the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. May be required for histone hyperacetylation. Involved in the estrogen-induced cell proliferation and cell cycle progression of breast cancer cells.

Product Categories/Family for anti-ATAD2 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 24497618
• NCBI GeneID: 29028
• NCBI Accession #: NP_054828; AB_2793609
• NCBI GenBank Nucleotide #: NM_014109
• UniProt Accession #: Q6PL18
• Molecular Weight: 153kDa
• NCBI Official Full Name: ATPase family AAA domain-containing protein 2
• NCBI Official Synonym Full Names: ATPase family AAA domain containing 2
• NCBI Official Symbol: ATAD2
• NCBI Official Synonym Symbols: ANCCA; CT137; PRO2000
• NCBI Protein Information: ATPase family AAA domain-containing protein 2
• UniProt Protein Name: ATPase family AAA domain-containing protein 2
• Protein Family: ATPase family AAA domain-containing protein
• UniProt Gene Name: ATAD2
• UniProt Synonym Gene Names: L16; PRO2000; ANCCA
• UniProt Entry Name: ATAD2_HUMAN

NCBI Description

A large family of ATPases has been described, whose key feature is that they share a conserved region of about 220 amino acids that contains an ATP-binding site. The proteins that belong to this family either contain one or two AAA (ATPases Associated with diverse cellular Activities) domains. AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes. The protein encoded by this gene contains two AAA domains, as well as a bromodomain. [provided by RefSeq, Jul 2008]