Immunohistochemistry (IHC)
(Anti-CDKL1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Mouse anti-Human CDKL1 Monoclonal Antibody | anti-CDKL1 antibody
Mouse Monoclonal [clone 1F12] (IgG2a,k) to Human CDKL1
Gene Names
CDKL1; P42; KKIALRE
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
Purity
Protein A Purified
Synonyms
CDKL1; Monoclonal Antibody; Mouse Monoclonal [clone 1F12] (IgG2a; k) to Human CDKL1; Anti-CDKL1 Antibody (clone 1F12) IHC-plus; CDC2-related kinase 1; KKIALRE; p42; Protein kinase p42 KKIALRE; Cyclin-dependent kinase-like 1; Human CDKL1; anti-CDKL1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1F12
Specificity
Human CDKL1
Purity/Purification
Protein A Purified
Form/Format
PBS, pH 7.2
Concentration
1 mg/ml (varies by lot)
Sequence Length
358
Applicable Applications for anti-CDKL1 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot with the recombinant protein used as the immunogen.
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot with the recombinant protein used as the immunogen.
Target Species
Human
Immunogen Description
CDKL1 (NP_004187, 259 a.a. ~ 358 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
CDKL1 antibody was raised against cDKL1 (NP_004187, 259 a.a. ~ 358 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Preparation and Storage
Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.
Immunohistochemistry (IHC)
(Anti-CDKL1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-CDKL1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to CDKL1 on HeLa cell. [antibody concentration 10 ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to CDKL1 on HeLa cell. [antibody concentration 10 ug/ml].)
ELISA (EIA)
(Detection limit for recombinant GST tagged CDKL1 is approximately 0.03 ng/ml as a capture antibody.)
ELISA (EIA)
(Detection limit for recombinant GST tagged CDKL1 is approximately 0.03 ng/ml as a capture antibody.)
Related Product Information for anti-CDKL1 antibody
CDKL1 belongs to the CDKL subfamily. CDKL1 has been observed to be up-regulated in gliosis. Multiple isoforms have been identified due to alternative splicing.
Product Categories/Family for anti-CDKL1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
108,931 Da
NCBI Official Full Name
cyclin-dependent kinase-like 1 isoform 1
NCBI Official Synonym Full Names
cyclin-dependent kinase-like 1 (CDC2-related kinase)
NCBI Official Symbol
CDKL1
NCBI Official Synonym Symbols
P42; KKIALRE
NCBI Protein Information
cyclin-dependent kinase-like 1; CDC2-related kinase 1; protein kinase p42 KKIALRE; serine/threonine protein kinase KKIALRE; serine/threonine-protein kinase KKIALRE
UniProt Protein Name
Cyclin-dependent kinase-like 1
Protein Family
UniProt Gene Name
CDKL1
UniProt Entry Name
CDKL1_HUMAN
NCBI Description
This gene product is a member of a large family of CDC2-related serine/threonine protein kinases. It accumulates primarily in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2013]
Uniprot Description
CDKL1: a protein kinase of the CDKL family. Accumulates primarily in the nucleus.
Protein type: Cell cycle regulation; Protein kinase, CMGC; EC 2.7.11.22; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDKL family
Chromosomal Location of Human Ortholog: 14q21.3
Cellular Component: cytoplasm; nucleus
Molecular Function: cyclin-dependent protein kinase activity; ATP binding
Biological Process: regulation of cell cycle; heart development; protein amino acid phosphorylation
Research Articles on CDKL1
Similar Products
Product Notes
The CDKL1 cdkl1 (Catalog #AAA249069) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 1F12] (IgG2a,k) to Human CDKL1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDKL1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA). ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB (1:500 - 1:1000) Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot with the recombinant protein used as the immunogen. Researchers should empirically determine the suitability of the CDKL1 cdkl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDKL1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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