Rabbit anti-Mouse CDC37 Polyclonal Antibody | anti-CDC37 antibody

Anti-CDC37 Antibody, Rabbit Polyclonal

Cat. Num. AAA8112115

• Gene Names: Cdc37; p50; p50Cdc37
• Reactivity: Mouse
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: CDC37; Polyclonal Antibody; Anti-CDC37 Antibody; Rabbit Polyclonal; CDC37 Antibody; Rabbit PAb; Antigen Affinity Purified; cell division cycle 37; Anti-p50 Antibody; Anti-p50Cdc37 Antibody; anti-CDC37 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse CDC37
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CDC37 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:2500-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Mouse CDC37 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse CDC37. CDC37 specific IgG was purified by Mouse CDC37 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse CDC37 in mouse brain with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse CDC37 in mouse intestine with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of mouse CDC37 in mouse kidney with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunoprecipitation (IP)

(Mouse CDC37 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-Mouse CDC37 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse CDC37 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size: 48 kDa)

Western Blot (WB)

(Anti-CDC37 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:42 kDaObserved band size:48 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-CDC37 antibody

CDC37 is a protein that is expressed in proliferative zones during embryonic development and in adult tissues, consistent with a positive role in proliferation and is required for cell division in budding yeast. CDC37 is thought to play an important role in the establishment of signaling pathways controlling cell proliferation through targeting intrinsically unstable oncoprotein kinases such as Cdk-4, Raf-1, and src to the molecular chaperone Hsp90. Decreased Hsp90 expression can reduce the levels of microtubule-associated protein tau, whose overexpression may induce many diseases. CDC37 is considered as a co-chaperone that is classified as Hsp90's accessory proteins. It has been reported that suppression of Cdc37 destabilized tau, leading to its clearance, whereas cdc37 overexpression preserved tau.Cdc37 was found to co-localize with tau in neuronal cells and to physically interact with tau from human brain. Moreover, Cdc37 levels significantly increased with age.

Product Categories/Family for anti-CDC37 antibody

AKT Signaling Pathway

References

Dai K, et al. (1996) P hysical Interaction of Mammalian CDC37 with CDK4. The journal of biological chemistry. 271: 22030-4.Pearl LH, et al. (2005) Hsp90 and Cdc37-a chaperone cancer conspiracy. Current opinion in genetics development. 15 (1): 55-61.Chen GQ, et al. (2002) TNF-Induced Recruitment and Activation of the IKK Complex Require Cdc37 and Hsp90. Molecular cell. 9 (2): 401-10.

NCBI and Uniprot Product Information

• NCBI GI #: 7949018
• NCBI GeneID: 12539
• NCBI Accession #: NP_058022.1
• NCBI GenBank Nucleotide #: NM_016742.4
• UniProt Accession #: Q61081; Q3TGP0
• Molecular Weight: 44,593 Da
• NCBI Official Full Name: hsp90 co-chaperone Cdc37
• NCBI Official Synonym Full Names: cell division cycle 37
• NCBI Official Symbol: Cdc37
• NCBI Official Synonym Symbols: p50; p50Cdc37
• NCBI Protein Information: hsp90 co-chaperone Cdc37; cell division cycle 37 homolog; hsp90 chaperone protein kinase-targeting subunit
• UniProt Protein Name: Hsp90 co-chaperone Cdc37
• Protein Family: Hsp90 co-chaperone
• UniProt Gene Name: Cdc37
• UniProt Entry Name: CDC37_MOUSE