Testing Data
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Nitro Blue Tetrazolium Substrate
Nitro Blue Tetrazolium (NBT) 0.489mM Staining System for AP
Applications
Immunohistochemistry
Purity
Molecular Biology Grade
Synonyms
Nitro Blue Tetrazolium; Nitro Blue Tetrazolium (NBT) 0.489mM Staining System for AP; Nitro Blue Tetrazolium substrate
Purity/Purification
Molecular Biology Grade
Form/Format
Colorless, pale yellow, complete
Applicable Applications for Nitro Blue Tetrazolium substrate
Immunohistochemistry (IHC)
pH
8.5 ± 0.1
Preparation and Storage
4 degree C
Stability: Stable at RT and 4 degree C
Stability: Stable at RT and 4 degree C
Testing Data
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Testing Data
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Related Product Information for Nitro Blue Tetrazolium substrate
Stable NBT solution useful in blotting assays for measuring dehydrogenases and in oxidase histochemistry for measuring oxygen and analyte detection systems that employ dehydrogenase enzyme chemistry. NBT has been employed extensively in dehydrogenase and oxidase chemistry. In the case of dehydrogenases, NAD or NADP along with intermediary electron acceptors/donors such as phenazine methosulfate, Meldola's blue and ferricyanide are employed. NADH or NADPH formed during the dehydrogenase reaction transfers electrons to the acceptor which in turn reduces NBT to an insoluble purple formazan. Enzymes such as xanthine oxidase and NADPH oxidase generate superoxide anion (O2-) which reduces NBT directly.
Product Categories/Family for Nitro Blue Tetrazolium substrate
Similar Products
Product Notes
The Nitro Blue Tetrazolium (Catalog #AAA639217) is a Substrate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Nitro Blue Tetrazolium can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the Nitro Blue Tetrazolium for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nitro Blue Tetrazolium, Substrate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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