CDC37 recombinant protein

CDC37 Protein, Mouse, Recombinant (His & GST Tag)

Cat. Num. AAA8123004

• Gene Names: Cdc37; p50; p50Cdc37
• Purity: >85% as determined by SDS-PAGE
• Synonyms: CDC37; CDC37 Protein; Mouse; Recombinant (His & GST Tag); Mouse CDC37/CDC37A Protein (His & GST Tag); p50 Protein; p50Cdc37 Protein; cell division cycle 37; CDC37 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala379

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the mouse CDC37 (Q61081)(Met1-Ala379) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CDC37 recombinant protein

Background: CDC37 is a protein that is expressed in proliferative zones during embryonic development and in adult tissues, consistent with a positive role in proliferation and is required for cell division in budding yeast. CDC37 is thought to play an important role in the establishment of signaling pathways controlling cell proliferation through targeting intrinsically unstable oncoprotein kinases such as Cdk-4, Raf-1, and src to the molecular chaperone Hsp90. Decreased Hsp90 expression can reduce the levels of microtubule-associated protein tau, whose overexpression may induce many diseases. CDC37 is considered as a co-chaperone that is classified as Hsp90's accessory proteins. It has been reported that suppression of Cdc37 destabilized tau, leading to its clearance, whereas cdc37 overexpression preserved tau.Cdc37 was found to co-localize with tau in neuronal cells and to physically interact with tau from human brain. Moreover, Cdc37 levels significantly increased with age.

Product Categories/Family for CDC37 recombinant protein

AKT Signaling Pathway

References

Dai K, et al. (1996) P hysical Interaction of Mammalian CDC37 with CDK4. The journal of biological chemistry. 271: 22030-4.Pearl LH, et al. (2005) Hsp90 and Cdc37-a chaperone cancer conspiracy. Current opinion in genetics development. 15 (1): 55-61.Chen GQ, et al. (2002) TNF-Induced Recruitment and Activation of the IKK Complex Require Cdc37 and Hsp90. Molecular cell. 9 (2): 401-10.

NCBI and Uniprot Product Information

• NCBI GI #: 7949018
• NCBI GeneID: 12539
• NCBI Accession #: NP_058022.1
• NCBI GenBank Nucleotide #: NM_016742.4
• UniProt Accession #: Q61081; Q3TGP0
• Molecular Weight: 44,593 Da
• NCBI Official Full Name: hsp90 co-chaperone Cdc37
• NCBI Official Synonym Full Names: cell division cycle 37
• NCBI Official Symbol: Cdc37
• NCBI Official Synonym Symbols: p50; p50Cdc37
• NCBI Protein Information: hsp90 co-chaperone Cdc37; cell division cycle 37 homolog; hsp90 chaperone protein kinase-targeting subunit
• UniProt Protein Name: Hsp90 co-chaperone Cdc37
• Protein Family: Hsp90 co-chaperone
• UniProt Gene Name: Cdc37
• UniProt Entry Name: CDC37_MOUSE