CADM2 recombinant protein

CADM2 Protein, Human, Recombinant (ECD, His Tag)

Cat. Num. AAA8121942

• Gene Names: CADM2; NECL3; IGSF4D; Necl-3; synCAM2; SynCAM 2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CADM2; CADM2 Protein; Human; Recombinant (ECD; His Tag); Human Cell Adhesion Molecule 2/CADM2 Protein (ECD; IGSF4D Protein; Necl-3 Protein; NECL3 Protein; SynCAM2 Protein; cell adhesion molecule 2; CADM2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly30-Asp335

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Gly 30
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CADM2 (NP_001161147.1)(Gly30-Asp335) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 264681535
• NCBI GeneID: 253559
• NCBI Accession #: NP_001161147.1
• NCBI GenBank Nucleotide #: NM_001167675.1
• UniProt Accession #: Q8N3J6; Q3KQY9; Q658Q7; Q8IZP8
• Molecular Weight: 47,554 Da
• NCBI Official Full Name: cell adhesion molecule 2 isoform 2
• NCBI Official Synonym Full Names: cell adhesion molecule 2
• NCBI Official Symbol: CADM2
• NCBI Official Synonym Symbols: NECL3; IGSF4D; Necl-3; synCAM2; SynCAM 2
• NCBI Protein Information: cell adhesion molecule 2; nectin-like 3; nectin-like protein 3; synaptic cell adhesion molecule 2; immunoglobulin superfamily member 4D; immunoglobulin superfamily, member 4D
• UniProt Protein Name: Cell adhesion molecule 2
• Protein Family: Cell adhesion molecule
• UniProt Gene Name: CADM2
• UniProt Synonym Gene Names: IGSF4D; NECL3; IgSF4D; NECL-3; SynCAM 2
• UniProt Entry Name: CADM2_HUMAN

NCBI Description

This gene encodes a member of the synaptic cell adhesion molecule 1 (SynCAM) family which belongs to the immunoglobulin (Ig) superfamily. The encoded protein has three Ig-like domains and a cytosolic protein 4.1 binding site near the C-terminus. Proteins belonging to the protein 4.1 family crosslink spectrin and interact with other cytoskeletal proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

CADM2: Adhesion molecule that engages in homo- and heterophilic interactions with the other nectin-like family members, leading to cell aggregation. Important for synapse organization, providing regulated trans-synaptic adhesion. Preferentially binds to oligodendrocytes. Belongs to the nectin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p12.1

Cellular Component: axon; cytoplasm; plasma membrane; integral to membrane; synapse; cell junction

Biological Process: intercellular junction assembly and maintenance; brain development; cell adhesion