Immunostaining
Rabbit CD68 Polyclonal Antibody | anti-CD68 antibody
CD68 Antibody
Gene Names
CD68; GP110; LAMP4; SCARD1
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Affinity Chromatography
Synonyms
CD68; Polyclonal Antibody; CD68 Antibody; Affinity Purified Rabbit Polyclonal CD68 Antibody; Polyclonal CD68 antibody; Anti-CD68 antibody; CD68 antigen antibody; Macrophage antigen CD68 antibody; Macrosialin antibody; CD 68 antibody; CD-68 antibody; SCARD1 antibody; GP110 antibody; CD 68; CD-68; Scavenger receptor class D member 1 antibody; anti-CD68 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Mouse, Rat
Purity/Purification
Affinity Chromatography
Form/Format
Lyophilized Form
Sequence Length
354
Applicable Applications for anti-CD68 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Frozen
Immunogen
CD68 antibody was raised in Rabbit using a synthetic peptide corresponding to a sequence in the middle region of Mouse CD68, different from the related rat sequence by one amino acid, as the immunogen
Post-Translational Modifications
CD68 is N- and O-glycosylated. Glycosylation occurs at Asn88, Asn96, Asn118, Asn126, Asn164, Asn199, Asn246, Asn261 and Asn279.
Expression
CD68 is highly expressed by blood monocytes and tissue macrophages. It is also expressed in lymphocytes, fibroblasts and endothelial cells. CD268 is located in the Lysosome membrane and the cellular membrane of most mammalian cells.
Preparation and Storage
Store at 4 degree C for short term storage. For long term storage aliquot and store at -20 degree C. Avoid repeated freeze/thaw cycles.
Immunostaining
Immunostaining
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded section of Rat Liver Tissue)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded section of Rat Liver Tissue)
Western Blot (WB)
(Lane 1: Rat Spleen Tissue Lysate; Lane 2: Rat Lung Tissue Lysate)
Western Blot (WB)
(Lane 1: Rat Spleen Tissue Lysate; Lane 2: Rat Lung Tissue Lysate)
Related Product Information for anti-CD68 antibody
Description: CD68 belongs to the LAMP family of proteins. It is composed of 354 amino acids with a molecular weight of 37408 Da. There is a Macrosialin chain at amino acids 22 - 354 and a Mucin-like region at aa 23 - 140.
Biological Significance: CD68 is a glycoprotein which binds to low density lipoprotein. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Implications In Disease: CD68 expression in many tumor cell lines could allow tumors to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. The presence of CD68 in macrophages also makes it useful in identifying conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease.
Biological Significance: CD68 is a glycoprotein which binds to low density lipoprotein. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Implications In Disease: CD68 expression in many tumor cell lines could allow tumors to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. The presence of CD68 in macrophages also makes it useful in identifying conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease.
Product Categories/Family for anti-CD68 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
CD68
NCBI Official Synonym Full Names
CD68 molecule
NCBI Official Symbol
CD68
NCBI Official Synonym Symbols
GP110; LAMP4; SCARD1
NCBI Protein Information
macrosialin
Protein Family
NCBI Description
This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]
Research Articles on CD68
Similar Products
Product Notes
The CD68 (Catalog #AAA5312829) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD68 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Frozen. Researchers should empirically determine the suitability of the CD68 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD68, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
