Testing Data
(Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Rat Anti-Mouse IgG1-PE (MBS675070))
IgG1 Secondary Antibody
Rat Anti-Mouse IgG1-BIOT
Reactivity
Mouse
Applications
ELISA, Flow Cytometry
Synonyms
IgG1; Monoclonal Secondary Antibody; Rat Anti-Mouse IgG1-BIOT; Rat Anti-Mouse IgG1; IgG1 secondary antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat (Sprague Dawley)IgG2bk
Clone Number
SB77e
Specificity
Mouse IgG1 Fc
Minimal reactivity to human, goat, and rabbit IgG and human, rat, hamster, bovine, horse, goat , sheep, chicken, guinea pig, donkey, and rabbit serum
Minimal reactivity to human, goat, and rabbit IgG and human, rat, hamster, bovine, horse, goat , sheep, chicken, guinea pig, donkey, and rabbit serum
Form/Format
Biotin (BIOT) Conjugate
Applicable Applications for IgG1 secondary antibody
ELISA, Flow Cytometry
Application Notes
Flow Cytometry:
FITC, BIOT, and AF488 conjugates - < ug/106 cells
PE and AF647 conjugates - <0.1 ug/106 cells
For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL.
ELISA:
AP conjugate - 1:1,000 - 1:4,000
HRP conjugate - 1:4,000 - 1:8,000
BIOT conjugate - 1:5,000-1:20,000
FLISA
FITC, AF488, and AF555 conjugates - 1:100 – 1:400
PE and AF647 conjugates - < 1ug/mL
Other Applications
Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
FITC, BIOT, and AF488 conjugates - < ug/106 cells
PE and AF647 conjugates - <0.1 ug/106 cells
For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL.
ELISA:
AP conjugate - 1:1,000 - 1:4,000
HRP conjugate - 1:4,000 - 1:8,000
BIOT conjugate - 1:5,000-1:20,000
FLISA
FITC, AF488, and AF555 conjugates - 1:100 – 1:400
PE and AF647 conjugates - < 1ug/mL
Other Applications
Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Preparation and Storage
The fluorescein (FITC) and biotin (BIOT) conjugates are supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8°C.
Testing Data
(Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Rat Anti-Mouse IgG1-PE (MBS675070))
Testing Data
(Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by Rat Anti-Mouse IgG1-PE (MBS675070))
Similar Products
Product Notes
The IgG1 (Catalog #AAA675070) is a Secondary Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse IgG1-BIOT reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IgG1 can be used in a range of immunoassay formats including, but not limited to, ELISA, Flow Cytometry. Flow Cytometry: FITC, BIOT, and AF488 conjugates - < ug/106 cells PE and AF647 conjugates - <0.1 ug/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL. ELISA: AP conjugate - 1:1,000 - 1:4,000 HRP conjugate - 1:4,000 - 1:8,000 BIOT conjugate - 1:5,000-1:20,000 FLISA FITC, AF488, and AF555 conjugates - 1:100 – 1:400 PE and AF647 conjugates - < 1ug/mL Other Applications Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need. Researchers should empirically determine the suitability of the IgG1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG1, Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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