Human Leukotriene A4 Hydrolase ELISA Kit | LTA4H elisa kit

Human Leukotriene A4 Hydrolase ELISA Kit

Cat. Num. AAA765116

• Reactivity: Human
• Synonyms: Leukotriene A4 Hydrolase; Human Leukotriene A4 Hydrolase ELISA Kit; LTA4H/EC 3.3.2.6/Leukotriene A (4) hydrolase/leukotriene A4 hydrolase/leukotriene A-4 hydrolase/LTA4/LTA-4 hydrolase; LTA4H elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of LTA4H. No significant cross-reactivity or interference between LTA4H and analogues was observed.
• Sequence Length: 611

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: <0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LTA4H were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LTA4H were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LTA4H elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- LTA4H antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- LTA4H antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the LTA4H amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of LTA4H can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 976396
• NCBI GeneID: 4048
• Molecular Weight: 66,852 Da
• NCBI Official Full Name: leukotriene A4 hydrolase
• NCBI Official Synonym Full Names: leukotriene A4 hydrolase
• NCBI Official Symbol: LTA4H
• NCBI Protein Information: leukotriene A-4 hydrolase
• UniProt Protein Name: Leukotriene A-4 hydrolase
• Protein Family: Leukotriene A-4 hydrolase
• UniProt Gene Name: LTA4H
• UniProt Synonym Gene Names: LTA4; LTA-4 hydrolase
• UniProt Entry Name: LKHA4_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

LTA4H: Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity. Belongs to the peptidase M1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - arachidonic acid; Hydrolase; EC 3.3.2.6

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; plasma membrane

Molecular Function: aminopeptidase activity; epoxide hydrolase activity; leukotriene-A4 hydrolase activity; peptidase activity; peptide binding; protein binding; zinc ion binding

Biological Process: arachidonic acid metabolic process; inflammatory response; leukotriene biosynthetic process; leukotriene metabolic process; peptide catabolic process; proteolysis; response to peptide hormone stimulus; response to zinc ion