Rabbit anti-Mouse CD40 Polyclonal Antibody | anti-CD40 antibody

Anti-CD40 antibody

Cat. Num. AAA175113

• Gene Names: Cd40; IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: CD40; Polyclonal Antibody; Anti-CD40 antibody; Tumor necrosis factor receptor superfamily member 5; CD40 molecule; TNF receptor superfamily member 5; AI326936 antibody; B cell associated molecule CD40 antibody; B cell surface antigen CD40 antibody; B cell-associated molecule antibody; B-cell surface antigen CD40 antibody; Bp50 antibody; CD 40 antibody; CD40 antibody; CD40 antigen(TNF receptor superfamily member 5) antibody; CD40 antigen antibody; CD40 molecule antibody; TNF receptor superfamily member 5 antibody; CD40 protein antibody; CD40 type II isoform antibody; CD40L receptor antibody; CDw40 antibody; GP39 antibody; HIGM1 antibody; IGM antibody; IMD3 antibody; MGC9013 antibody; Nerve growth factor receptor related B lymphocyte activation molecule antibody; OTTHUMP00000031699 antibody; OTTHUMP00000031700 antibody; p50 antibody; T-BAM antibody; TBAM antibody; TNFRSF5 antibody; TNR5_HUMAN antibody; TRAP antibody; Tumor necrosis factor receptor superfamily; member 5 antibody; Tumor necrosis factor receptor superfamily member 5 antibody; Tumor necrosis factor receptor superfamily member 5 precursor antibody; member 5; isoform CRA_a antibody; anti-CD40 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity wit other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 289

• Applicable Applications for anti-CD40 antibody: Western Blot (WB)
• Application Notes: Western Blot ; ; Concentration: 0.1-0.5 ug/mL; Tested Species: Mouse; ; ; Tested Species: In-house tested species with positive results.; Predicted Species: Species predicted to be fit for the product based on sequence similarities.; ; Other applications have not been tested.; Optimal dilutions should be determined by end users.
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse CD40 (22-36aa, QCVTCSDKQYLHDGQ).
• Reconstitution: 0.2 ml of distilled water with yield a concentration of 500 ug/mL.
• Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃
• Preparation and Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-CD40 antibody, MBS175113, Western blottingLane 1: Recombinant Mouse CD 40 Protein 10ngLane 2: Recombinant Mouse CD 40 Protein 5ngLane 3: Recombinant Mouse CD 40 Protein 2.5ng )

Related Product Information for anti-CD40 antibody

Background: CD40 is a cell surface receptor that is expressed on the surface of all mature B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor(TNF) receptor superfamily and is the receptor for CD40 ligand. CD40 ligand(CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.

NCBI and Uniprot Product Information

• NCBI GI #: 172046819
• NCBI GeneID: 21939
• UniProt Accession #: P27512; Q3TS33; Q3TSL2; Q3U799; Q3U7C9; Q3UBH3; Q542B1; Q8K2X6; Q99NE0; Q99NE1; Q99NE2; Q99NE3
• NCBI Official Full Name: Tumor necrosis factor receptor superfamily member 5
• NCBI Official Synonym Full Names: CD40 antigen
• NCBI Official Symbol: Cd40
• NCBI Official Synonym Symbols: IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 5; CD40L receptor; B-cell surface antigen CD40; T-cell differentiation antigen; tumor necrosis factor receptor superfamily, member 5
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 5
• Protein Family: CD40 ligand
• UniProt Gene Name: Cd40
• UniProt Synonym Gene Names: Tnfrsf5
• UniProt Entry Name: TNR5_MOUSE

Uniprot Description

CD40: a member of the TNF-receptor superfamily. This receptor for CD40L mediates a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. Defects in CD40 are the cause of hyper-IgM immunodeficiency type 3 (HIGM3). HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. Two alternatively spliced isoforms have been reported. Isoform I is a type I membrane protein; isoform II is secreted.

Protein type: Membrane protein, integral; Receptor, cytokine

Cellular Component: extracellular space; cell surface; membrane; intracellular membrane-bound organelle; cell; cytoplasm; integral to membrane; plasma membrane; extracellular region; external side of plasma membrane

Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; antigen binding

Biological Process: positive regulation of isotype switching to IgG isotypes; regulation of immune response; B cell activation; immune system process; positive regulation of interleukin-12 production; immune response-regulating cell surface receptor signaling pathway; activation of NF-kappaB transcription factor; cellular calcium ion homeostasis; positive regulation of MAP kinase activity; positive regulation of tyrosine phosphorylation of Stat1 protein; protein kinase B signaling cascade; positive regulation of B cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; inflammatory response; defense response to virus; regulation of immunoglobulin secretion