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Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Skin stained with 8-oxoguanine Mouse Monoclonal Antibody (CPTC-OGG1-1).)

Mouse anti-Human 8-Oxoguanine DNA Glycosylase Monoclonal Antibody | anti-OGG1 antibody

8-Oxoguanine DNA Glycosylase

Gene Names
OGG1; HMMH; MUTM; OGH1; HOGG1
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
8-Oxoguanine DNA Glycosylase; Monoclonal Antibody; HMMH; HOGG1; MUTM; OGH1; anti-OGG1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2c, kappa
Clone Number
CPTC-OGG1-1
Specificity
8-oxoguanine (8-oxoG), an oxidized form of guanine, is produced by reactive oxygen species in both DNA and nucleotide pools during normal aging. Accumulation of 8-oxoG increases the occurrence of A:T to C:G or G:C to T:A transversionmutations, because 8-oxoG forms a stable basepair with adenine as well as with cytosine. OGG1 (for 8-oxoG DNA glycosylase), also designated MMH, is a DNA repair enzyme that corrects these mutations. Inactivation of the OGG1 gene leads to a mutator phenotype, characterized by the increase in G:C to T:A transversions. The OGG1 gene encodes eight isoforms (OGG1A-C, OGG2A-E) which result from alternative splicing of a single messenger RNA. The OGG1A splice variant is the most prevalent form and localizes to the nucleus, whereas the OGG2A splice variant is targeted to the mitochondria. Guanine is the main target for reactive oxygen species in DNA, and 8-oxoguanine is the most frequent base lesion. Therefore, formation of 8-oxoguanine is an important biomarker of oxidative damage to DNA. It is primarily repaired by the DNA glycosylase OGG1. Furthermore, defects in OGG1 may be a cause of renal cell carcinoma.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
190
Applicable Applications for anti-OGG1 antibody
Immunohistochemistry (IHC) Paraffin
Application Notes
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Positive Control
HeLa or Jurkat cells. Kidney, skin or lymph node.
Immunogen
Recombinant full-length human OGG1 protein
Cellular Localization
Mitochondrion. Nucleus speckle.
Hu-chromosome Location
3p25.3
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Non-hazardous.
No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Skin stained with 8-oxoguanine Mouse Monoclonal Antibody (CPTC-OGG1-1).)

Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Skin stained with 8-oxoguanine Mouse Monoclonal Antibody (CPTC-OGG1-1).)

SDS-Page

(SDS-PAGE Analysis Purified 8-oxoguanine Mouse Monoclonal Antibody (CPTC-OGG1-1). Confirmation of Purity and Integrity of Antibody.)

SDS-Page (SDS-PAGE Analysis Purified 8-oxoguanine Mouse Monoclonal Antibody (CPTC-OGG1-1). Confirmation of Purity and Integrity of Antibody.)
References
Cheng, K.C., et al. 1992. J. Biol. Chem. 267: 166-17

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
39kDa
NCBI Official Full Name
OGG1 protein type 1b, partial
NCBI Official Synonym Full Names
8-oxoguanine DNA glycosylase
NCBI Official Symbol
OGG1
NCBI Official Synonym Symbols
HMMH; MUTM; OGH1; HOGG1
NCBI Protein Information
N-glycosylase/DNA lyase
UniProt Protein Name
N-glycosylase/DNA lyase
Protein Family
UniProt Gene Name
OGG1
UniProt Synonym Gene Names
MMH; MUTM; OGH1; AP lyase
UniProt Entry Name
OGG1_HUMAN

NCBI Description

This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008]

Uniprot Description

OGG1: DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N- methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta- lyase activity that nicks DNA 3' to the lesion. Defects in OGG1 may be a cause of renal cell carcinoma (RCC). It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. Belongs to the type-1 OGG1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Deoxyribonuclease; EC 4.2.99.18; DNA repair, damage

Chromosomal Location of Human Ortholog: 3p26.2

Cellular Component: nucleoplasm; nuclear matrix; mitochondrion; nuclear speck

Molecular Function: protein binding; endonuclease activity; microtubule binding; oxidized purine base lesion DNA N-glycosylase activity; DNA N-glycosylase activity; damaged DNA binding; 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity

Biological Process: response to drug; depurination; DNA repair; DNA catabolic process, endonucleolytic; response to estradiol stimulus; response to radiation; response to ethanol; base-excision repair, AP site formation; regulation of transcription, DNA-dependent; base-excision repair; nucleotide-excision repair; response to folic acid; regulation of protein import into nucleus, translocation; response to oxidative stress; acute inflammatory response; negative regulation of apoptosis; aging

Disease: Renal Cell Carcinoma, Nonpapillary

Research Articles on OGG1

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Product Notes

The OGG1 ogg1 (Catalog #AAA4381139) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The 8-Oxoguanine DNA Glycosylase reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's 8-Oxoguanine DNA Glycosylase can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin. Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the OGG1 ogg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "8-Oxoguanine DNA Glycosylase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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